http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Go, Yun Young,Rajapakse, R. P. V. Jayanthe,Kularatne, Senanayake A. M.,Lee, Pei-Yu Alison,Ku, Keun Bon,Nam, Sangwoo,Chou, Pin-Hsing,Tsai, Yun-Long,Liu, Yu-Lun,Chang, Hsiao-Fen Grace,Wang, Hwa-Tang Tho American Society for Microbiology 2016 Journal of clinical microbiology Vol.54 No.6
<P>Dengue virus (DENV) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. Here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal PCR (RT-iiPCR) method for the detection of all four serotypes of DENV in clinical samples. The diagnostic performance of the newly established pan-DENV RT-iiPCR assay targeting a conserved 3' untranslated region of the viral genome was evaluated. The limit of detection with a 95% confidence was estimated to be 10 copies of in vitro-transcribed (IVT) RNA. Sensitivity analysis using RNA prepared from 10-fold serial dilutions of tissue culture fluid containing DENVs suggested that the RT-iiPCR assay was comparable to the multiplex real-time quantitative RT-PCR (qRT-PCR) assay for DENV-1, -3, and -4 detection but 10-fold less sensitive for DENV-2 detection. Subsequently, plasma collected from patients suspected of dengue virus infection (n = 220) and individuals not suspected of dengue virus infection (n = 45) were tested by the RT-iiPCR and compared to original test results using a DENV NS1 antigen rapid test and the qRT-PCR. The diagnostic agreement of the pan-DENV RT-iiPCR, NS1 antigen rapid test, and qRT-PCR tests was 93.9%, 84.5%, and 97.4%, respectively, compared to the composite reference results. This new RT-iiPCR assay along with the portable POCKIT nucleic acid analyzer could provide a highly reliable, sensitive, and specific point-of-need diagnostic assay for the diagnosis of DENV in clinics and hospitals in developing countries.</P>
Chern-Sheng Lin,Jen Min Tsai,Kai-Sheng Chen,Yun-Long Lay,Nin-Chun Chang 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.
In this paper an automatic optical inspection system for the advanced fiber coupler assembly manufacturing process is presented. Coupling efficiency variations were compared with axial displacement. The main standard components of light peak fiber couplerswere used in the experiment to determine the corresponding offset and position of the optimal optical coupling efficiency in order toachieve a positioning effect. This study used the parameters of lens curvature, focal length and light source for validation using opticaldesign software. An infrared CCD camera was used to extract the coupled light spot images. The curve fitting method and couplingsimulation database were used for image processing and to calculate optimal coupling efficiency. Under optimal coupling efficiency,the minimum amount of movement of the translation stages was used to calculate the offset of each axle or the coupling efficiencywhen tilted. Moreover, a coupling efficiency database was established. The optical axle center offset in the alignment was calculatedby comparing the data in the database. Finally, the optical axle center offset or tilt direction was identified through the program toachieve the position of optimal coupling efficiency.