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        Sulphur dioxide plasma modification on poly(methyl methacrylate) for fluidic devices

        Atsunori Hiratsuka,Hiroyuki Fukui,Yoshio Suzuki,Hitoshi Muguruma,Koji Sakairi,Toshiyuki Matsushima,Yuji Maruo,Kenji Yokoyama 한국물리학회 2008 Current Applied Physics Vol.8 No.2

        We developed a sulphur dioxide plasma modication on a poly(methyl methacrylate) (PMMA) material for uidic electrophoresisdevices. The inner surface of the PMMA channel of a chip was modied by using sulphur dioxide plasma treatment. Contact angle mea-surements indicated that the buer solutions were able to ll the capillary because of the hydrophilic property of the internal surface ofthe chip. XPS analysis indicated that the sulphur dioxide plasma treatment introduced a negative charge originating from the dissoci-ation of the hydrogen atoms of sulphonic groups. Since this introduced a negative charge originating from strong acid sulphonic groupsonto the surface of the channel, the electroosmotic ow (EOF) was observed to be large (~7×10-⁴m²V-¹ s-¹) and stable over a widerange of pH (410). The chip was fabricated by using plastic injection moulding methods for enabling the mass fabrication and dispos-able use of chips. The separation method is based on the net electric charge of the material, which enables the separation of identicalsamples on the basis of both isoelectric points and molecular weight. Two synthetic peptides with similar isoelectric points and molecularweights but dierent net charges were selected as model protein samples for the separation. The sample peptides were detected underuorescence microscopy. The resulting electropherograms obtained by using the sulphur dioxide plasma-treated PMMA chip demon-strated that the two peptides were separated and that the migration time of the peptides was correlated with the net charge.

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