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- 저자 : Yoshiaki Sugaya (검색결과 1 건)
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Toshiro Ohashi,Yoshiaki Sugaya,Naoya Sakamoto,Masaaki Sato 대한의용생체공학회 2016 Biomedical Engineering Letters (BMEL) Vol.6 No.1
Purpose Vascular endothelial cells (ECs) are continuouslysubjected to mechanical forces such as fluid shear stress,stretching and hydrostatic pressure. The effect of hydrostaticpressure on EC responses has not been fully understoodcompared to that of the other two stimuli. The purpose ofthis study is to assess mechanical responses of ECs to thesemechanical stimuli. Methods Bovine aortic ECs were exposed to hydrostaticpressure of 50, 100, and 150 mmHg and fluid shear stressof 3 Pa in simultaneous or successive fashion. Immunofluorescencestaining of actin filaments and VEcadherin wasthen performed to observe cell morphology and cell-celljunctions, respectively. Results The results showed that ECs subjected to 50, 100,and 150 mmHg for 24 h elongated without predominantorientation and exhibited multilayered structure, whereassimultaneous application of 50 and 100 mmHg and 3 Pa for24 h induced marked elongation and orientation of ECsparallel to the direction of flow maintaining monolayerintegrity. This monolayer integrity was lost in ECs subjectedto 150 mmHg together with 3 Pa. A successive applicationof 100 mmHg for 24 h followed by 100 mmHg and 3 Pa for24 h, indicated that the loss of monolayer integrity due tohydrostatic pressure could not be retrieved by the followingsimultaneous application. Conclusions It can be concluded that physiological shearstress of 3 Pa is dominant to physiological hydrostatic pressureup to 100 mmHg, importantly suggesting the relativecontribution of physiological hydrostatic pressure and fluidshear stress to endothelial monolayer integrity.
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