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      • KCI등재

        Source-Sink Partitioning of Mineral Nutrients and Photo-assimilates in Tomato Plants Grown under Suboptimal Nutrition

        Sung, Jwakyung,Lee, Suyeon,Lee, Yejin,Yun, Hongbae,Ha, Sangkeun,Ok, Yongsik Korean Society of Soil Science and Fertilizer 2013 한국토양비료학회지 Vol.46 No.6

        A huge number of greenhouse soils in Korea have accumulated mineral elements which induce many nutritional and pathological problems. The present study was performed to the effects of the reduced fertilization on plant growth, and uptake and partitioning of minerals (N, P, K) and soluble carbohydrates using highly minerals-accumulated farmer's greenhouse soil. On the basis of the recommended application for tomato crop, the application rates of N, P and K were 110(50%)-5.2(5%)-41.5(35%)kg $ha^{-1}$, respectively, using Hoagland's nutrient solution. Tomato growth rates during the whole experiment were not significant between treatments, but it was found that a decrease in daily growth represented after 60 days of treatment (DAT). The reduced application led to a drastic decrease in the concentration of N, P and K in fruits, and, thus, this resulted in lower uptake after 40 DAT. The lower phloem export and utilization of soluble carbohydrates caused an accumulation of extra-carbohydrates in leaves, stems and fruits in the reduced application. The reduced fertilization induced the capture of N, P and K in leaves and of soluble carbohydrates in stems compared to the conventional application. In this study, we suggest that it is possible to delay the first fertigation time in minerals-accumulated soils without an adverse impact on crop growth, but it is necessary to regularly monitor mineral status in soil to ensure a balanced uptake, synthesis and partitioning of minerals and carbohydrates.

      • KCI등재

        Temporal Changes in N Assimilation and Metabolite Composition of Nitrate-Affected Tomato Plants

        Sung, Jwakyung,Lee, Suyeon,Lee, Yejin,Kim, Rogyoung,Lee, Juyoung,Lee, Jongsik,Ok, Yongsik Korean Society of Soil Science and Fertilizer 2012 한국토양비료학회지 Vol.45 No.6

        The role of inorganic nitrogen assimilation in the production of amino acids, organic acids and soluble sugars is one of the most important biochemical processes in plants, and, in order to achieve normally, nitrate uptake and assimilation is essential. For this reason, the characterization of nitrate assimilation and metabolite composition from leaves, roots and xylem sap of tomato (Solanum lycopersicum) was investigated under different nitrate levels in media. Tomato plants were grown hydroponically in liquid culture under five different nitrate regimes: deficient (0.25 and 0.75 mM $NO_3{^-}$), normal (2.5 mM $NO_3{^-}$) and excessive (5.0 and 10.0 mM $NO_3{^-}$). All samples, leaves, roots and xylem sap, were collected after 7 and 14 days after treatment. The levels of amino acids, soluble sugars and organic acids were significantly decreased by N-deficiency whereas, interestingly, they remained higher in xylem sap as compared with N-normal and -surplus. The N-excessive condition did not exert any significant changes in metabolites composition, and thus their levels were similar with N-normal. The gene expression and enzyme activity of nitrate reductase (NR), nitrite reductase (NIR) and glutamine synthetase (GS) were greatly influenced by nitrate. The data presented here suggest that metabolites, as a signal messenger, existed in xylem sap seem to play a crucial role to acquire nitrate, and, in addition, an increase in ${\alpha}$-ketoglutarate pathway-derived amino acids under N-deficiency may help to better understand plant C/N metabolism.

      • KCI등재

        Source-Sink Partitioning of Mineral Nutrients and Photo-assimilates in Tomato Plants Grown under Suboptimal Nutrition

        Jwakyung Sung,Suyeon Lee,Yejin Lee,Hongbae Yun,Sangkeun Ha,Yongsik Ok 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6

        A huge number of greenhouse soils in Korea have accumulated mineral elements which induce many nutritional and pathological problems. The present study was performed to the effects of the reduced fertilization on plant growth, and uptake and partitioning of minerals (N, P, K) and soluble carbohydrates using highly minerals-accumulated farmer’s greenhouse soil. On the basis of the recommended application for tomato crop, the application rates of N, P and K were 110(50%)-5.2(5%)-41.5(35%)kg ha<SUP>-1</SUP>, respectively, using Hoagland’s nutrient solution. Tomato growth rates during the whole experiment were not significant between treatments, but it was found that a decrease in daily growth represented after 60 days of treatment (DAT). The reduced application led to a drastic decrease in the concentration of N, P and K in fruits, and, thus, this resulted in lower uptake after 40 DAT. The lower phloem export and utilization of soluble carbohydrates caused an accumulation of extra-carbohydrates in leaves, stems and fruits in the reduced application. The reduced fertilization induced the capture of N, P and K in leaves and of soluble carbohydrates in stems compared to the conventional application. In this study, we suggest that it is possible to delay the first fertigation time in minerals-accumulated soils without an adverse impact on crop growth, but it is necessary to regularly monitor mineral status in soil to ensure a balanced uptake, synthesis and partitioning of minerals and carbohydrates.

      • KCI등재

        Temporal Changes in N Assimilation and Metabolite Composition of Nitrate-Affected Tomato Plants

        Jwakyung Sung,Suyeon Lee,Yejin Lee,Rogyoung Kim,Juyoung Lee,Jongsik Lee,Yongsik Ok 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.6

        The role of inorganic nitrogen assimilation in the production of amino acids, organic acids and soluble sugars is one of the most important biochemical processes in plants, and, in order to achieve normally, nitrate uptake and assimilation is essential. For this reason, the characterization of nitrate assimilation and metabolite composition from leaves, roots and xylem sap of tomato (Solanum lycopersicum) was investigated under different nitrate levels in media. Tomato plants were grown hydroponically in liquid culture under five different nitrate regimes: deficient (0.25 and 0.75 mM NO<sub>3</sub> -), normal (2.5 mM NO<sub>3</sub> -) and excessive (5.0 and 10.0 mM NO<sub>3</sub> -). All samples, leaves, roots and xylem sap, were collected after 7 and 14 days after treatment. The levels of amino acids, soluble sugars and organic acids were significantly decreased by N-deficiency whereas, interestingly, they remained higher in xylem sap as compared with N-normal and -surplus. The N-excessive condition did not exert any significant changes in metabolites composition, and thus their levels were similar with N-normal. The gene expression and enzyme activity of nitrate reductase (NR), nitrite reductase (NIR) and glutamine synthetase (GS) were greatly influenced by nitrate. The data presented here suggest that metabolites, as a signal messenger, existed in xylem sap seem to play a crucial role to acquire nitrate, and, in addition, an increase in α-ketoglutarate pathway-derived amino acids under N-deficiency may help to better understand plant C/N metabolism.

      • KCI등재

        Physiological Responses of Tomato Plants and Soil Microbial Activity in Salt Affected Greenhouse Soil

        Sung, Jwakyung,Lee, Suyeon,Nam, Hyunjung,Lee, Yejin,Lee, Jongsik,Almaroai, Yaser A.,Ok, Yongsik Korean Society of Soil Science and Fertilizer 2012 한국토양비료학회지 Vol.45 No.6

        Crop productivity decreases globally as a result of salinization. However, salinity impact on greenhouse-grown crops is much higher than on field-grown crops due to the overall concentrations of nutrients in greenhouse soils. Therefore, this study was performed to determine the short-term changes in growth, photosynthesis, and metabolites of tomato plants grown in greenhouse under heavily input of fertilizers evaluated by microbial activity and chemical properties of soils. The soils (< 3, 3.01~6, 6.01~10 and > 10.01 dS $m^{-1}$) from farmer's greenhouse fields having different fertilization practices were used. Results showed that the salt-accumulated soil affected adversely the growth of tomato plants. Tomato plants were seldom to complete their growth against > 10.0 dS $m^{-1}$ level of EC. The assimilation rate of $CO_2$ from the upper fully expanded leaves of tomato plants is reduced under increasing soil EC levels at 14 days, however; it was the highest in moderate or high EC-subjected (3.0 ~ 10.0 dS $m^{-1}$) at 28 days. In our experiment, soluble sugars and starch were sensitive markers for salt stress and thus might assume the status of crops against various salt conditions. Taken together, tomato plants found to have tolerance against moderate soil EC stress. Various EC levels (< 3.0 ~ 10.0 dS $m^{-1}$) led to a slight decrease in organic matter (OM) contents in soils at 28 days. Salinity stress led to higher microbial activity in soils, followed by a decomposition of OM in soils as indicated by the changes in soil chemical properties.

      • KCI등재

        Physiological Responses of Tomato Plants and Soil Microbial Activity in Salt Affected Greenhouse Soil

        Jwakyung Sung,Suyeon Lee,Hyunjung Nam,Yejin Lee,Jongsik Lee,Yaser. A. Almaroai,Yongsik Ok 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.6

        Crop productivity decreases globally as a result of salinization. However, salinity impact on greenhouse-grown crops is much higher than on field-grown crops due to the overall concentrations of nutrients in greenhouse soils. Therefore, this study was performed to determine the short-term changes in growth, photosynthesis, and metabolites of tomato plants grown in greenhouse under heavily input of fertilizers evaluated by microbial activity and chemical properties of soils. The soils (< 3, 3.01~6, 6.01~10 and >10.01 dS m<sup>-1</sup>) from farmer’s greenhouse fields having different fertilization practices were used. Results showed that the salt-accumulated soil affected adversely the growth of tomato plants. Tomato plants were seldom to complete their growth against > 10.0 d Sm<sup>-1</sup> level of EC. The assimilation rate of CO2 from the upper fully expanded leaves of tomato plants is reduced under increasing soil EC levels at 14 days, however; it was the highest in moderate or high EC-subjected (3.0 ~ 10.0 dS m<sup>-1</sup>) at 28 days. In our experiment, soluble sugars and starch were sensitive markers for salt stress and thus might assume the status of crops against various salt conditions. Taken together, tomato plants found to have tolerance against moderate soil EC stress. Various EC levels (<3.0 ~ 10.0 dS m<sup>-1</sup>) led to a slight decrease in organic matter (OM) contents in soils at 28 days. Salinity stress led to higher microbial activity in soils, followed by a decomposition of OM in soils as indicated by the changes in soil chemical properties.

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