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RISS 인기검색어
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- 저자 : Yingjian Lu (검색결과 2 건)
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Fengxia Lv,Chong Zhang,Fangfang Guo,Yingjian Lu,Xiaomei Bie,Hui Qian,Zhaoxin Lu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.1
The gene encoding the fibrinolytic enzyme (PPFE-I) from Paenibacillus polymyxa EJS-3 was cloned and sequenced (Genbank No. KC176802). The 1773 bp gene encoded 590 amino acids and had low homology with other known fibrinolytic enzymes. PPFE-I was soluble and expressed in E. coli BL21 to enhance the activity. The recombinant enzyme (rPPFE-I) was purified to homogeneity, and enzymatic properties were characterized. The optimal temperature and pH for rPPFE-I were 37℃ and 7.5, respectively. Observed activities of rPPFE-I were highest in the presence of Zn2+, Mg2+, and Fe2+ and the lowest in the presence of Ca2+ and Cu2+. The rPPFE-I activity was strongly inhibited by PMSF. The α-chain was affected by the rPPFE-I cleavage speed of fibrinogen, followed by the b and γ chains. The inhibitory effects of rPPFE-I against ADP-induced human platelet aggregation were dosedependent with an IC50 value of 1.54 μM.
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Zhou Zhe,Zhang Ming,Xu Mingxi,Zhang Ke,Zhao Yang,Zhou Juan,Zhu Yingjian,Wang Zhong,Lu Mujun 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
The objective of this study was to investigate whether intraperitoneal incubation improves the regenerative capacity of bladder acellular matrix grafts (BAMGs) in a rat model of bladder augmentation. After 2 weeks of incubation in the peritoneum of male rats, BAMG flaps with vascular pedicles were harvested for autologous bladder augmentation. As the control, BAMGs were directly used for bladder augmentation without intraperitoneal incubation. Histological analyses of the incubated BAMGs demonstrated extensive cell growth and vasculature in homogeneous collagen bundles. The cells were positive for vimentin and negative for α-smooth muscle actin and pan-cytokeratin AE1/AE3. Cystography revealed smoother contours of the augmented bladders in the incubated group at 4 and 12 weeks postoperatively. However, the bladder capacity was not significantly different between the two groups. In both groups, the entire urothelium regenerated well without obvious differences. At both time points, compared with the control group, increased numbers of smooth muscle cells (SMCs) and blood vessels were found in the incubated group. At 12 weeks, the SMCs in the incubated group were more similar to those in the native smooth muscle fiber bundles of the bladder. Taken together, our results demonstrated that BAMGs preincubated in the peritoneum promote the regeneration of bladder smooth muscle via neovascularization in a rat bladder augmentation model.
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