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Xiaoman Li,Wenchao Zhao,Xiaoxuan Zhou,Jiaping Feng,Yingjian Gao,Xuehui Yao,Yang Liu,Jie Liu,Rui Yang,Fukuan Zhao,Shaohui Wang 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.6
Root-knot nematodes (RKNs) (Meloidogyne spp.) are one of the most damaging crop pests and cause severeyield losses worldwide. Easier and more efficient methods are needed for assessing nematode infestations and hostplant resistance. In the present study, we combined toluidine blue staining and paraffin sectioning to observe theestablishment of giant cells (GCs; redifferentiated cells induced by nematode feeding) and the developmental status ofRKNs in tomato (Solanum lycopersicum). The number of RKNs was counted using a modified freeze-thaw countingmethod. Finally, three tomato lines with different levels of RKN resistance, but otherwise identical genetic backgrounds,were tested to verify the applicability of the combined method. The results suggested that using toluidine blue stainingcombined with paraffin sectioning and freeze-thaw counting accurately reflects the level of RKN resistance of tomato plants.