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DNA hypermethylation level of ACTL6A may promote BmNPV infection in B. mori
Chen Zikang,Huang Haoling,Yin Haotong,Shen Manman,Guo Xijie,Wuping Liao 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
Bombyx mori nucleopolyhedrosis virus (BmNPV) can cause acute virus disease in silkworm, seriously endan gering the sustainable development of sericultural industry. DNA methylation is an extensive regulation mode of gene expression in epigenetics, which plays an important role in a series of life processes such as genomic imprinting, embryo development, aging and viral infection. Previously, we found the DNA hypermethylation of actin-like protein 6A gene (ACTL6A) in BmNPV-infected midgut of silkworm larvae by whole genome bisulfite sequencing. In this study, firstly, we validated the enhanced transcription level and the DNA methylation level of ACTL6A in the midgut upon BmNPV infection by qRT-PCR and BS-PCR, respectively. Then, treated the BmN cells with DNA methylation inhibitor (Zebularine), both the DNA methylation level and the transcription level of ACTL6A were declined. Further, knock down of ACTL6A by siRNA inhibited the proliferation of BmNPV. The results showed that BmNPV infection may induce the DNA hypermethylation of ACTL6A in Bombyx mori, which promoted the transcription level of ACTL6A, and then affected the proliferation of BmNPV. Our results may provide an insight into exploring the role of DNA methylation in the interaction between BmNPV and silkworm.
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