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Jieqin Li,Yihua Wang,Juntao Chai,Lihua Wang,Chunming Wang,Wuhua Long,Di Wang,Yunlong Wang,Ming Zheng,Cheng Peng,Mei Niu,Jianmin Wan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.5
The nuclear genes involved in chloroplast developmentand chlorophyll biosynthesis must be investigated tounderstand their functions in plant growth and development. In this study, we isolated and identified a unique leaf-colormutant of rice with a green-yellow phenotype before thefour-leaf stage and named the mutation green-revertiblechlorina 1 (grc1). The mutants had significantly lower plantheight, number of tillers, and panicle length and headedsignificantly earlier than the wild type. The levels ofchlorophylls, carotenoids, and chlorophyll precursors werealso lower. The mutation in grc1 affected chloroplastultrastructure, particularly thylakoid development. Geneticanalysis indicated that the green-yellow phenotype wascontrolled by a single recessive gene. We mapped the grc1gene to a 32.4-kb region on the long arm of chromosome 6. Through map-based cloning, we identified a 45-bp insertionin the genomic region of LOC_Os06g40080, which encodeda heme oxygenase. Expression of LOC_Os06g40080 wassignificantly down-regulated in the grc1 mutant. Subcellularlocalization showed that this heme oxygenase was localizedin the chloroplast. In summary, we isolated and identified thegene for grc1, which plays an important role in chlorophyllbiosynthesis and chloroplast development in rice.
Jia Lyu,Yihua Wang,Linglong Liu,Chunming Wang,Yulong Ren,Cheng Peng,Feng Liu,Yunlong Wang,Mei Niu,Di Wang,Ming Zheng,Kunneng Zhou,Shaolu Zhao,Fuqing Wu,Haiyang Wang,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
Differentiation from proplastids into chloroplasts isa light- and energy-dependent process. How this process isregulated is still poorly understood at the molecular level. We herein report a new putative plastidial adenine nucleotidetransporter, BRITTLE1-3 (referred to as OsBT1-3), encoded bythe rice (Oryza sativa) White Stripe Leaf 2 (WSL2) gene. Loss of OsBT1-3 function results in defective chloroplastbiogenesis, severely reduced photosynthetic efficiency, andfinally a white stripe leaf phenotype in the first four leaves. The expression levels of genes related to chlorophyllbiosynthesis and photosynthesis are drastically reduced,accompanied with over accumulation of reactive oxygenspecies (ROS) in the wsl2 mutant. OsBT1-3 is targeted tothe chloroplasts and it expresses in almost all tissues inplants, especially in young leaves. OsBT1-3 consists of 419amino acids and exhibits features of all mitochondrialcarrier proteins, including a typical transmembrane-spanningdomain and a highly conserved sequence motif designatedas the ‘mitochondrial energy transfer signatures’. Phylogeneticanalysis shows that OsBT1-3 is a putative plastidialadenine nucleotide transporter and is most closely relatedto ZmBT1-2. Together, these observations suggest that thenew putative adenine nucleotide transporter, OsBT1-3,plays an essential role in regulating chloroplast biogenesisand maintenance of ROS homeostasis during rice seedlingde-etiolation.
Shaolu Zhao,Wuhua Long,Yihua Wang,Linglong Liu,Yunlong Wang,Mei Niu,Ming Zheng,Di Wang,Jianmin Wan 한국식물학회 2016 Journal of Plant Biology Vol.59 No.3
Leaf-color mutants are ideal genetic materials for understanding the mechanism of chloroplast development and chlorophyll (Chl) biosynthesis. Here we isolated and identified a new leaf-color mutant of rice, named white-stripe leaf3 (wsl3), from a 60Co-irradiated mutant pool. The wsl3 mutant displayed a visible white-stripe leaf in both young seedlings and flag leaves of mature plant. Chl content in homozygous wsl3 mutant was approximately 47% of that in the wild type. Besides, chloroplast development in the mutant was severely arrested. By a map-based cloning strategy, the wsl3 gene was finely confined to a 50.8 kb region on chromosome 1. Moreover, a 9-bp deletion was identified in the genomic region of LOC_Os01g01920, which encodes an HD (histidine and aspartic acid) domaincontaining protein. Genetic complementation confirmed that LOC_Os01g01920 could recover the lesion of wsl3 mutation. Real-time PCR analyses showed that the expression levels of WSL3 were the highest in young and flag leaves among various tissues, and most of the genes associated with Chl biosynthesis were significantly down-regulated in the wsl3 mutant. Meanwhile, in contrast to many nuclear gene-encoded phage-type RNA polymerase(s) (NEP) transcribed genes were up-regulated, most of plastid-encoded bacterialtype RNA polymerase (PEP) transcribed genes were downregulated. These results demonstrated that the WSL3 gene, as an HD domain-containing protein, is involved in chl biosynthesis and chloroplast development in rice.
Qizhang Long,Wenwei Zhang,Peng Wang,Wenbiao Shen,Tong Zhou,Nannan Liu,Ren Wang,Ling Jiang,Jiexue Huang,Yihua Wang,Yuqiang Liu,Jianmin Wan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.4
Lipoxygenases (LOXs) are enzymes involved in lipid peroxidation. Here we reported the identification, molecular and functional characterization of the gene encoding rice (Oryza sativa L.) seed LOX3 (sLOX3). Via a map-based cloning strategy we identified Os03g0700400 as the candidate gene encoding sLOX3. Further functional complementary test and biochemical characterization of the recombinant Os03g0700400 protein verified the identification. The sLOX3 gene was highly expressed in roots, moderately in embryos and very weakly in leaves, leaf sheaths and stems. Transient expression experiment (in rice protoplasts) and subsequent laser confocal microscopic analysis demonstrated that the sLOX3 protein was localized into the cytosol. We next showed that overexpression of sLOX3 in a japonica sLOX3-normal rice cultivar, Wuyunjing 7 accelerated the decrease of seed germination ability when the seeds were routinely stored, which demonstrated that sLOX3 had a negative effect on seed longevity (storability). Meanwhile, an increased occurrence of embryo decay was observed in the same transgenic seeds, suggesting that sLOX3 might negatively affect seed longevity by facilitating colonization of particular seed pathogens. Our result forwarded the understanding of the effects of 9-LOX on rice seed longevity.
Mingsheng Zhong,Xi Liu,Feng Liu,Yulong Ren,Yunlong Wang,Jianping Zhu,Xuan Teng,Erchao Duan,Fan Wang,Huan Zhang,Mingming Wu,Yuanyuan Hao,Xiaopin Zhu,Ruonan Jing,Xiuping Guo,Ling Jiang,Yihua Wang,Jianmi 한국식물학회 2019 Journal of Plant Biology Vol.62 No.1
Starch is a major storage substance in cerealgrains, and starch biosynthesis is a complex process. In orderto elucidate regulation of the starch biosynthesis pathway, wescreened a series of rice (Oryza sativa L.) endospermmutants. In this study, we identified a floury white-coreendosperm mutant named floury endosperm12 (flo12). Theflo12 mutant exhibited loosely packed starch granules and alower thousand kernel weight compared to wild type. Semithinsections revealed that compound starch grains (SG) inflo12 interior endosperm cells were developed abnormally. Furthermore, amylose content was decreased, while totalprotein content was significantly increased in flo12 grains. Map-based cloning showed that FLO12 encodes rice alanineaminotransferase 1 (OsAlaAT1). OsAlaAT1 is highly expressedin developing endosperm. Subcellular localization showedthat OsAlaAT1 is localized in the cytosol. Moreover, theexpression of most starch synthesis-related genes wasdecreased, while most of the storage protein coding geneshad elevated expression levels in the flo12 mutant. Inaddition, overexpression of the OsAlaAT1 gene increasedgrain weight. In brief, we demonstrated that OsAlaAT1regulates carbon and nitrogen metabolism, which provides anew insight for the improvement of rice quality and yield.
Yihua Zhang,Yuan Wang,Jiao Li,Baoxi Cai 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.10
With Fujian Province as object of study, panel data from 1980 to 2013 is selected in this paper. Besides, VAR model is established. Co-integration test, Granger causality, impulse response function and variance decomposition are used for quantitative analysis on correlation between real estate development, financial expenditure and economic growth in Fujian Province. The research results show that long-term stable balance among real estate development, financial expenditure and economic growth in Fujian Province. Real estate development and financial expenditure are the Granger factors driving the growth of economic growth. Financial expenditure is the Granger reason for real estate development’s development. On this basis, the author proposes some policies and recommendations for macroeconomic regulation and control in Fujian Province.
Yihua Zhang,Yuan Wang,Lufen Guo,Baoxi Cai 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.7
Based on economic growth theory of modern economic theory, with Fujian Province as object of study, panel data from 1981 to 2013 is selected in this paper. Besides, VAR model is established. Co-integration test, Granger causality and impulse response function are used for quantitative analysis on correlation between consumption, investment, import, export and economic growth in Fujian Province. With Eviews8 software as analytical tool, the empirical study conducts analysis from the perspective of econometrics and tests the stationarity of unit root data; determines optimal lag intervals through VAR model analysis on different lag intervals; determines the existence of co-integration relationship of data through Johansen co-integration test; tests the causality of data with Granger causality test; conducts stationarity test by establishing VAR model; studies the variation trend of influence of consumption, investment and import and export on economic growth in the next ten years through impulse response. The author reveals long-term stable balance among consumption, investment, import, export and economic growth in Fujian Province as well as bidirectional causal relationship between consumption and economic growth. For a short term, household consumption is of relatively strong positive impact effect on economic gain, which is served as major impetus of economic growth. Domestic investment is of relatively obvious promotion for economic growth in a short term and of certain negative effect on economic growth in a long term. Import and export have significant function for promoting economic growth and profound influence in Fujian as coastal city. On this basis, the author proposes some policies and recommendations for macroeconomic regulation and control in Fujian Province.
Research on Dialectic Relation between Marine Industrial Structure and Macro Economic Growth
Yuan Wang,Yihua Zhang,Xichun Ge 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.9
With Fujian Province as object of study, panel data from 1981 to 2013 is selected in this paper. Besides, VAR model is established. Co-integration test, Granger causality and impulse response function are used for quantitative analysis on correlation between the three main marine industries and economic growth in Fujian Province. In the paper, the long-term stable relation between the three main marine industries and economic growth is revealed, and the causal relations between the primary marine industry and economic growth, between the secondary marine industry and economic growth and between tertiary marine industry and economic growth are verified. The government should give priority to the secondary marine industry and effective support to the tertiary marine industry when adjusting the industrial structure and optimizing policies.