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RISS 인기검색어
- 검색키워드
- 저자 : Yamindago Ade (검색결과 3 건)
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Impact of zinc oxide nanoparticles on the bacterial community of Hydra magnipapillata
Ade Yamindago,Nayun Lee,Seonock Woo,Seungshic Yum 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1
Backgrounds Zinc oxide nanoparticles (ZnO NPs) are extensively used for various products. In this study, the effects of ZnO NPs exposure in diversity and community composition of the bacteria associated with H. magnipapillata were investigated. This study provides insight into possible impacts of ZnO NPs on aquatic organisms. Methods 454-pyrosequencing analysis of the bacterial 16S rRNA gene was applied to H. magnipapillata after exposure to 10 mg/L ZnO NPs (Ø 20 nm). Results Acute exposure to ZnO NPs changed the diversity and compositions of the associated bacteria. The composition of Curvibacter decreased, but Flectobacillus and Delftia increased; these two genera are known to have beneficial functions. Conclusion The changes in diversity and composition of the associated bacteria may indicate the possible mechanisms by which the associated bacteria maintain their mutual interactions and support the health of their host after exposure to ZnO NPs.
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Acute toxic effects of zinc oxide nanoparticles on <i>Hydra magnipapillata</i>
Yamindago, Ade,Lee, Nayun,Woo, Seonock,Choi, Hyosun,Mun, Ji Young,Jang, Seok-Won,Yang, Sung Ik,Anton-Erxleben, Friederike,Bosch, Thomas C.G.,Yum, Seungshic Elsevier 2018 Aquatic toxicology Vol.205 No.-
<P><B>Abstract</B></P> <P>Zinc oxide nanoparticles (ZnO NPs) are increasingly used in various products as coating and additive materials for household goods, personal-care products, and drug delivery systems. Because of their broad applications, the potential risks to nontarget organisms associated with their input into aquatic environments have generated much concern. We investigated the acute toxicity, morphological responses, and potential impact on physiology and metabolism in polyps exposed to spherical ZnO NPs of either 20 nm (ZnO NP<SUB>20</SUB>) or 100 nm (ZnO NP<SUB>100</SUB>). The median lethal concentrations (LC<SUB>50</SUB>) of ZnO NP<SUB>20</SUB> were 55.3, 8.7, and 7.0 μg/mL after exposure for 48, 72, and 96 h, respectively; and those of ZnO NP<SUB>100</SUB> were 262.0, 14.9, and 9.9 μg/mL, respectively. The morphological responses of the hydra polyps to a range of ZnO NP concentrations suggest that ZnO NPs may negatively affect neurotransmission in <I>Hydra</I>. ZnO NPs may also induce abnormal regeneration in the polyps by affecting the expression of several genes related to the Wnt signaling pathway. The presence of ZnO NP<SUB>20</SUB> in the hydra tissue was confirmed with electron microscopy. A Gene Ontology analysis of the genes differentially expressed in hydra polyps after exposure to ZnO NP<SUB>20</SUB> for 12 or 24 h revealed changes in various processes, including cellular and metabolic process, stress response, developmental process, and signaling. A KEGG pathway analysis of hydra polyps after exposure of ZnO NP<SUB>20</SUB> or ZnO NP<SUB>100</SUB> for 12 or 24 h demonstrated various changes, including in the DNA replication and repair, endocytosis, lysosomes, Wnt signaling, and natural killer-cell-mediated cytotoxicity pathways, suggesting the mechanisms that maintain cellular homeostasis in response to ZnO NPs. Progesterone-mediated oocyte maturation was also affected by the ZnO NPs nanoparticles, suggesting that they are potential endocrine disruptors. This study should increase our concern regarding the dispersal of ZnO NPs in aquatic environments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Toxicity of the Zinc oxide nanoparticles (ZnO NPs) is influenced by the size of the particles and the period of exposure. </LI> <LI> ZnO NPs induce changes in the morphology and abnormal regeneration in <I>H. magnipapillata.</I> </LI> <LI> ZnO NPs penetrated and accumulated in the cells of <I>H. magnipapillata.</I> </LI> <LI> ZnO NPs induce cytotoxic and genotoxic responses. </LI> </UL> </P>
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Transcriptome dynamics in benzo[a]pyrene exposed Hydra
Lee Nayun,Woo Seonock,Lee Nayoung,Jo Yejin,Yamindago Ade,Yum Seungshic 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.3
Backgrounds Benzo[a]pyrene (BaP) is a well-known ecotoxicant that induces a wide spectrum of toxic effects in organisms, including carcinogenicity, teratogenicity, genotoxicity, and immunotoxicity. Thus, re-evaluation of its acute toxic effects in gene expression at sublethal concentration in experimental animal is essential to understand and/or predict metabolic and physiological changes in an organism after exposure. Objectives To understand the changes in acute toxicity by exposure time, differential gene expression profiling of Hydra magnipapillata was performed by DNA microarray after exposure to BaP. Results The median lethal concentrations of the animals (LC 50 ) were determined to be 78.5, 53.6, and 28.9 mg/L after exposure to BaP for 24, 48, and 72 h, respectively. Morphological responses of hydra polyps to 50 mg/L of BaP by exposure time were observed. The gene expression levels of molecular chaperones, antioxidative enzymes were altered at the early phase of the exposure. Transcription of the genes related to development and differentiation; apoptosis and necrosis were affected by the 4 h BaP exposure group. After 12 h exposure, developmental processes, immune responses, and ion transport in hydra polyp seemed to be affected, since the transcriptions of the genes that related to those biological processes were induced or repressed by BaP exposure. Neurotransmission might be suppressed, but tumorigenesis and carcinogenesis, the DNA repair process might be induced in the 24 h BaP-exposed hydra group. Finally, tumorigenesis and carcinogenesis, and the DNA repair process seemed to be induced after 48 h exposure. Conclusion We successfully demonstrated the dynamic response of Hydra to BaP by exposure time at transcription level. The results could extend our knowledge on acute toxic effect of BaP at sublethal conditions.
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