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        Effects of Exogenous Organic Acids on the Growth and Antioxidant System of Cosmos bipinnatus under Cadmium Stress

        Jiani Hu,Xinyi Gao,Yajie Yue,Xiaoyu Ming,Linjie Yue,Lijuan Yang,Xue Xiao,Xiaofang Yu 한국원예학회 2022 원예과학기술지 Vol.40 No.5

        Cadmium pollution in China’s urban green spaces is extremely severe, causing a significant increase in the risk of urban environmental pollution. Applying organic acids under Cd stress conditions improves the activity of the major antioxidant enzymes in the AsA-GSH cycle signi- ficantly. In this study, Cosmos bipinnatus seedlings were cultured in a nutritive medium containing 40 µmol·L-1 Cd. They were subsequently supplemented with different concentrations of citric acid and malic acid (0, 0.5, 1.0, 2.0 mmol L-1 ) to analyze the roles of the organic acids in regulating the AsA-GSH cycle involved in plant resistance to Cd stress. Exogenous organic acids inhibited the growth of C. bipinnatus in a dose-dependent manner. AsA, GSH, and GR in the leaves decreased dramatically at high malic acid concentrations (2 mol L-1 ). The contents of GSH and GR and the APX activities showed a downward trend when the seedlings were treated with 0.5–2 mmol·L-1 of citric acid. However, AsA exhibited contrasting results under these conditions. Notably, both the antioxidant and enzyme activities were reduced considerably to a minimum when the citric acid concentration was 2 mmol·L-1 . Low concentrations of exogenous acids increased the activities of APX and GR in the leaves of C. bipinnatus, thus increasing the contents of ASA and GSH. Such increases boost the efficiency of the ASA-GSH cycle in relieving the toxic effect of cadmium on C. bipinnatus. Cadmium pollution in China’s urban green spaces is extremely severe, causing a significant increase in the risk of urban environmental pollution. Applying organic acids under Cd stress conditions improves the activity of the major antioxidant enzymes in the AsA-GSH cycle signi- ficantly. In this study, Cosmos bipinnatus seedlings were cultured in a nutritive medium containing -1 40 µmol·L Cd. They were subsequently supplemented with different concentrations of citric acid -1 and malic acid (0,0.5,1.0,2.0 mmol L ) to analyze the roles of the organic acids in regulating the AsA-GSH cycle involved in plant resistance to Cd stress. Exogenous organic acids inhibited the growth of C. bipinnatus in a dose-dependent manner. AsA, GSH, and GR in the leaves decreased -1 dramatically at high malic acid concentrations (2 mol L ). The contents of GSH and GR and the -1 APX activities showed a downward trend when the seedlings were treated with 0.5–2 mmol·L of citric acid. However, AsA exhibited contrasting results under these conditions. Notably, both the antioxidant and enzyme activities were reduced considerably to a minimum when the citric acid -1 concentration was 2 mmol·L . Low concentrations of exogenous acids increased the activities of APX and GR in the leaves of C. bipinnatus, thus increasing the contents of ASA and GSH. Such increases boost the efficiency of the ASA-GSH cycle in relieving the toxic effect of cadmium on C. bipinnatus.

      • Discovery of nitrate–CPK–NLP signalling in central nutrient–growth networks

        Liu, Kun-hsiang,Niu, Yajie,Konishi, Mineko,Wu, Yue,Du, Hao,Sun Chung, Hoo,Li, Lei,Boudsocq, Marie,McCormack, Matthew,Maekawa, Shugo,Ishida, Tetsuya,Zhang, Chao,Shokat, Kevan,Yanagisawa, Shuichi,Sheen, Nature Publishing Group, a division of Macmillan P 2017 Nature Vol.545 No.7654

        Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca<SUP>2+</SUP> signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca<SUP>2+</SUP>-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of cpk10 cpk30 cpk32 triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional cpk10 cpk30 cpk32 and nlp7 mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca<SUP>2+</SUP> signalling network integrates transcriptome and cellular metabolism with shoot–root coordination and developmental plasticity in shaping organ biomass and architecture.

      • SCOPUSSCIE

        Structure and Electrical Performance of Na<sub>2</sub>C<sub>6</sub>O<sub>6</sub> under High Pressure

        Wang, Xuan,Zhang, Peijie,Tang, Xingyu,Guan, Junjie,Lin, Xiaohuan,Wang, Yajie,Dong, Xiao,Yue, Binbin,Yan, Jinyuan,Li, Kuo,Zheng, Haiyan,Mao, Ho-kwang American Chemical Society 2019 The Journal of Physical Chemistry Part C Vol. No.

        <P>Sodium rhodizonate (Na<SUB>2</SUB>C<SUB>6</SUB>O<SUB>6</SUB>) has very high theoretical capacity as a positive electrode material of sodium-ion batteries, but it still has problems such as low actual capacity and poor electronic/ionic conductivity. In order to improve its conductivity, we investigated its structure and electrical properties under high pressure. By performing in situ X-ray diffraction, Raman, infrared absorption, and alternating current impedance spectroscopy in the range of 0-30 GPa at room temperature, we observed a phase transition at ∼11 GPa, with the conductivity increasing by an order of magnitude. Above ∼20 GPa, Na<SUB>2</SUB>C<SUB>6</SUB>O<SUB>6</SUB> gradually amorphized. During the decompression process, the pressure regulation of the structure and properties of the material are reversible. Our study shows that applying external pressure is an effective tool to improve the conductivity of molecular battery materials. The investigation will help to obtain next-generation electrode materials.</P> [FIG OMISSION]</BR>

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