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- 저자 : Xunhong Liu (검색결과 1 건)
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Fengsu Zhang,Hao Cai,Xunhong Liu,Fei Chen,Nianyun Yang,Ya Hou,Yang Ma,Yiyuan Luo,Juanxiu Liu 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.9
To analyze the components of the fatty oilextracted from Antrodia camphorata fungus powder and toevaluate the antioxidant activity of A. camphorata crudeoil. A derivatized gas chromatography mass spectrometry(GC–MS) was developed for the quantification of 19 fattyacids in extracts of A. camphorata obtained by supercriticalcarbon dioxide (SC-CO2). Good separation was obtainedunder the optimized chromatographic conditions and 19target compounds after methyl-esterification were identifiedby GC–MS on a HP-VOC capillary column(60 m 9 320 lm 9 1.8 lm) with an initial temperatureset at 80 C. The validity of the established method wasexamined experimentally with good linearity, intra-assayprecisions, repeatability, stability and recovery. The antioxidantactivity of A. camphorata crude oil was evaluatedby 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavengingassay. Nineteen fatty acids showed good linearityover the tested ranges (r[0.9956) and the recovery rangedfrom 93.47 to 104.89 %. The crude oil extracted from A. camphorata fungus powder also revealed its antioxidantactivity. It was the report about simultaneous determinationof 19 fatty acids in A. camphorata and antioxidant activityof its crude oil for the first time. The established methodmight also be utilized for the investigation of edible plantmaterials and agricultural products containing fatty acids.
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