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Xuancheng He,Feiyan Xue,Lulu Zhang,Hui-Li Guo,Lan-Qing Ma,Mingfeng Yang 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.5
Resveratrol (trans-3,4′,5-trihydroxystilbene) is a phytoalexin produced in plants in response to pathogen attack as a part of plant defense response and it is also a highly bioactive substance of pharmaceutical interest. To obtain transgenic plants with a high level of resveratrol, two enzymes in the last two steps of resveratrol synthesis, 4-coumaroyl-CoA ligase (4CL) and stilbene synthase (STS), were fused together by a glycine–serine–glycine (GSG) tripeptide linker, and the 4CL-GSGSTS construct driven by a CaMV35S promoter was transformed into tobacco (Nicotiana benthamiana) by Agrobacteriummediated method. In the transgenic plants, a high resveratrol level was detected (21.05 μg/g fresh weight) by high-pressure liquid chromatography (HPLC), which is higher than previous transgenic plants with only STS gene overexpression. In addition to resistance to pathogen, transgenic plants showed improved tolerance to salt and osmotic stresses, and the lower level of malondialdehyde (MDA) in transgenic plants suggested that resveratrol could protect plant membrane lipid from peroxidation under abiotic stresses.