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Li Chaochao,Xu Xinhui,Liu Shenghao,Zhang Pengying 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1
Chalcone synthase (CHS), a key enzyme in plant flavonoid synthesis, is essential for plant tolerance to abiotic stress. However, little research on CHS from the earliest terrestrial plants, such as mosses, has been reported. Here, the biological function of a CHS gene from Antarctic moss Pohlia nutans (PnCHS1) was studied. PnCHS1 had a 32.8–53.7% similarity to CHS from other species, however it still had highly conserved motifs of CHS such as Catalytic site (Asn366, His333) and Co-A binding site (Ser146). Subcellular localization analysis showed that PnCHS1 was distributed in the cell membrane and in the membranes of endothelial organelles. Heterologous expression of PnCHS1 increased flavonoid content in 5-day-old Arabidopsis grown with 24 h light and 17-day-old Arabidopsis cultured with sucrose, as well as anthocyanin content in the latter. PnCHS1 heterologous expression in Arabidopsis increased plant tolerance to salt stress, including a high germina- tion rate and a long taproot. Heterologous expression of PnCHS1 boosted tolerance to oxidative stress while decreasing the sensitivity to ABA. Under H2O2 or ABA stress, the expression pattern of PnCHS1, ROS scavenging enzyme gene (FeSOD1, FeSOD2, Cu-Zn-SOD2, and Cu-Zn-SOD3) and three genes of ABA signal pathway (RAB18, RD29B, and NCED3) were considerably up-regulated by real-time quantitative analysis. It indicates that PnCHS1 could enhance plant tolerance to NaCl and oxidative stresses, and may play a role in the adaptation of Antarctic moss to extreme environments.
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Mechanism analysis of pressure fluctuation in wheel loader articulated steering hydraulic system
Bing-wei Cao,Xinhui Liu,Wei Chen,Chen Zhou,Fei-xiang Xu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.10
This paper studies the influence of hinge point positioning of a steering cylinder on the pressure fluctuation of the steering system of a wheel loader. Affected by the hinge point position of the steering cylinder, the main causes of the pressure fluctuation are found to be the stroke difference and the force arm difference. Based on theoretical analysis, the hinge point positioning of the steering cylinder was optimized by using genetic algorithm (GA). The AMEsim simulation model of the steering system was built to verify the accuracy of the GA. The wheel loader was reformed according to the optimized articulated points position. Steering tests showed a significant drop in system pressure fluctuation, the energy consumed is also reduced. Further, the analysis shows that the force arm difference is the main factor causing the pressure fluctuation, providing the theoretical and experimental basis for the design of a steering system of wheel loaders.
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