http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Xinping Jia,Yanming Deng,Xiaobo Sun,Lijian Liang,Xiaoqing Ye 한국유전학회 2015 Genes & Genomics Vol.37 No.1
Seashore paspalum (Paspalum vaginatum O. Swartz) is a halophytic warm-season perennial grass,which plays an important role in protection of environment. However, very limited transcriptomic and genomic informationare currently available for seashore paspalum. Inthis study, the seashore paspalum transcriptome weresequenced using Illumina paired-end sequencing technology. In total, 32,603 unigenes with an average length of970 bp were obtained by de novo assembly. Of the unigenes,25,411 unigenes (77.94 %) had significant similaritywith known proteins in the NCBI non-redundant proteinand Swiss-Prot databases. Of these annotated unigenes,20,962 and 10,620 unigenes were assigned to gene ontologycategories and clusters of orthologous groups,respectively. A total of 4,699 unigenes were mapped into118 Kyoto Encyclopedia of Genes and Genomes pathways. In addition, 3,010 potential simple sequence repeats (SSRs)were predicted for microsatellite analyse. Tri-nucleotidewas the dominant repeat (1,583, 52.58 %), followed by dinucleotide(975, 32.39 %) and tetra-nucleotide (217,7.21 %). Fifty SSR sites were randomly selected for validationand development of EST–SSR markers. This studyprovided the global sequence data for seashore paspalumand demonstrated that the Illumina paired-end sequencingis a fast and cost-effective approach to gene discovery andmolecular marker development.