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        Experimental observation of oil mist penetration ability in minimum quantity lubrication (MQL) spray

        Guangyuan Zhu,Songmei Yuan,Xiaoyao Kong,Chong Zhang,Bochuan Chen 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.8

        Minimum quantity lubrication (MQL) is an eco-friendly cooling and lubricating method that has its importance in machining sustainability. However, the behavior of the MQL droplets remains questionable. The present study is conducted to investigate the penetration performance of MQL with different combination of spray parameters and capillary sizes. An observation platform has been designed, which makes the overall penetration process controllable. The results have shown that the shortest penetration time was obtained with larger capillary size, higher air flow rate and the corresponding smaller droplet size. However, the increase of air flow rate has the optimal value when the objective is to penetrate to 1 mm deep from the entrance, whereas it has not shown the similar regulation when the droplet flowed deeper to the center due to the long transport distance has amplified the effects of multiple influence factors. The observation results were verified through simulation and cutting experiment.

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        Fungal diversity in deep-sea sediments from Magellan seamounts environment of the western Pacific revealed by high-throughput Illumina sequencing

        Shuai Yang,Wei Xu,Yuanhao Gao,Xiaoyao Chen,Zhu-Hua Luo 한국미생물학회 2020 The journal of microbiology Vol.58 No.10

        There are lots of seamounts globally whose primary production is disproportionally greater than the surrounding areas. Compared to other deep-sea environments, however, the seamounts environment is relatively less explored for fungal diversity. In the present study, we explored the fungal community structure in deep-sea sediments from four different stations of the Magellan seamounts environment by using high-throughput sequencing of the ITS1 region. A total of 1,897,618 ITS1 sequences were obtained. Among these sequences, fungal ITS1 sequences could be clustered into 1,662 OTUs. The majority of these sequences belonged to Ascomycota. In the genera level, the most abundant genus was Mortierella (4.79%), which was reported as a common fungal genus in soil and marine sediments, followed by Umbelopsis (3.80%), Cladosporium (2.98%), Saccharomycopsis (2.53%), Aspergillus (2.42%), Hortaea (2.36%), Saitozyma (2.20%), Trichoderma (2.12%), Penicillium (2.11%), Russula (1.86%), and Verticillium (1.40%). Most of these recovered genera belong to Ascomycota. The Bray-Curtis analysis showed that there was 37 to 85% dissimilarity of fungal communities between each two sediment samples. The Principal coordinates analysis clearly showed variations in the fungal community among different sediment samples. These results suggested that there was a difference in fungal community structures not only among four different sampling stations but also for different layers at the same station. The depth and geographical distance significantly affect the fungal community, and the effect of depth and geographical distance on the structure of the fungal community in the Magellan seamounts is basically same. Most of the fungi were more or less related to plants, these plant parasitic/symbiotic/endophytic fungi constitute a unique type of seamounts environmental fungal ecology, different from other marine ecosystems.

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