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Wang Zengsheng,Li Yan,Kuang Xiaochuan,Guo Fang,Lang Tao,Mao Min,Zhang Xiaoyan,Yang Haiqing 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.3
Background MicroRNAs (miRNAs) function as post-transcriptional mediators for genes involved in cancer progression, including chronic lymphocytic leukemia. MiR-582-5p has been identified as a tumor suppressor in various tumors. The antioncogenic role of miR-582-5p was then validated in this study. Objective Mononuclear cells were isolated from peripheral blood samples of patients with chronic lymphocytic leukemia and healthy donors. Expression of miR-582-3p in the mononuclear cells was examined by qRT-PCR. CCK8 assay was performed to detect cell viability, and cell cycle and apoptosis were evaluated by flow cytometry. Dual luciferase activity assay was performed to determine the targeting relationship between miR-582-3p and HNRNPA1, and western blot was performed to unravel the mechanism. Results MiR-582-5p was reduced in mononuclear cells of patients with chronic lymphocytic leukemia compared to healthy donors. Forced miR-582-5p expression reduced cell viability, and promoted apoptosis of chronic lymphocytic leukemia cells. Cell cycle was arrested in G0/G1 phase via miR-582-5p mimic. MiR-582-5p bound to HNRNPA1 (heterogeneous nuclear ribonucleoprotein A1) and down-regulated its expression. Silence of HNRNPA1 decreased cell viability, promoted apoptosis, and blocked cell cycle at G0/G1 phase through up-regulation of IκBα (IkappaBalpha). Moreover, HNRNPA1 silencing attenuated the promotive effect induced by miR-582-5p inhibitor on the progression of chronic lymphocytic leukemia. Conclusion MiR-582-5p demonstrated anti-proliferative and pro-apoptotic roles in chronic lymphocytic leukemia cell growth via down-regulation of HNRNPA1 and up-regulation of IκBα, thus inactivating NF-κB. Background MicroRNAs (miRNAs) function as post-transcriptional mediators for genes involved in cancer progression, including chronic lymphocytic leukemia. MiR-582-5p has been identified as a tumor suppressor in various tumors. The antioncogenic role of miR-582-5p was then validated in this study. Objective Mononuclear cells were isolated from peripheral blood samples of patients with chronic lymphocytic leukemia and healthy donors. Expression of miR-582-3p in the mononuclear cells was examined by qRT-PCR. CCK8 assay was performed to detect cell viability, and cell cycle and apoptosis were evaluated by flow cytometry. Dual luciferase activity assay was performed to determine the targeting relationship between miR-582-3p and HNRNPA1, and western blot was performed to unravel the mechanism. Results MiR-582-5p was reduced in mononuclear cells of patients with chronic lymphocytic leukemia compared to healthy donors. Forced miR-582-5p expression reduced cell viability, and promoted apoptosis of chronic lymphocytic leukemia cells. Cell cycle was arrested in G0/G1 phase via miR-582-5p mimic. MiR-582-5p bound to HNRNPA1 (heterogeneous nuclear ribonucleoprotein A1) and down-regulated its expression. Silence of HNRNPA1 decreased cell viability, promoted apoptosis, and blocked cell cycle at G0/G1 phase through up-regulation of IκBα (IkappaBalpha). Moreover, HNRNPA1 silencing attenuated the promotive effect induced by miR-582-5p inhibitor on the progression of chronic lymphocytic leukemia. Conclusion MiR-582-5p demonstrated anti-proliferative and pro-apoptotic roles in chronic lymphocytic leukemia cell growth via down-regulation of HNRNPA1 and up-regulation of IκBα, thus inactivating NF-κB.
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Lei Cai,Chao CHEN,Wenjun Wang,Xiu Gao,Xiaoyan Kuang,Yan Jiang,Liang Li,Guoqiang Wu 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.91 No.-
Titanium silicalite-1 (TS-1) zeolite was successfully synthesized by hydrothermal method in the presenceof the tetrapropylammonium hydroxide (TPAOH) template, and a series of TS-1 supported cadmium withdifferent Cd loading (xCd/TS-1) catalysts were carefully prepared to produce epoxidized soybean oil(ESO) through the acid-free catalytic epoxidation of soybean oil (SO) with H2O2. These catalysts were fullycharacterized using various modern techniques and further evaluated systematically in this epoxidationreaction to understand the relationship between catalytic activity and structure of active components. The results show that the highly dispersed Cd species are introduced on the TS-1 support withoutaffecting the coordination environment of tetrahedral Ti (framework Ti). Furthermore, the Cd species arecoordinated with tetrahedral Ti species via O atom resulting in the weakness of the Ti–O bonds and facileattack of the Ti–O bonds by alcohol and H2O2, which can easily form thefive-membered intermediate andpromote the SO epoxidation process. Furthermore, the deactivation and regeneration behaviors of 2%Cd/TS-1 catalyst in SO epoxidation were also investigated, which reveal that the catalyst deactivation of cokedeposition is due to the strong adsorption of the catalysts and SO molecules. But the spent 2%Cd/TS-1 canbe completely regenerated by calcination method.
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