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He, Bangxiang,Hou, Lulu,Zhang, Feng,Cong, Xiaomei,Wang, Zhendong,Guo, Yalin,Shi, Jiawei,Jiang, Ming,Zhang, Xuecheng,Zang, Xiaonan The Korean Society of Phycology 2020 ALGAE Vol.35 No.3
Haematococcus pluvialis is a commercial microalga that can produce high quantities of astaxanthin. Under induced conditions, some important changes in the subcellular structures related to astaxanthin accumulation were observable. For example, a large number of astaxanthin granules, oil structures and starch granules appeared in the thick-walled cells; Astaxanthin granules gradually dissolved into the oil structures and spread throughout the entire cell with the fusion and diffusion process of oil structures during the middle and late stages of induction; The plastoglobules were closed to the newly formed structures, and some plastoglobules would abnormally increase in size under stress. Based on observations of cell damage, the degradation of membrane structures, such as chloroplasts, was found to be the primary form of damage during the early stage of induction. During the middle stage of induction, some transparent holes were exposed in the dissolving astaxanthin granules in the cytoplasm. In thick-walled cells, these transparent holes were covered by oil substances dissolving astaxanthin, thereby avoiding further damage to cells. Given the relatively few oil structures, in non-thick-walled cells, the transparent holes expanded to form multiple transparent areas, eventually resulting in the rupture and death of cells. These results suggested that the high level of synthesis and the wide range diffusion of oil explained the expansion of astaxanthin in H. pluvialis.
He-yun Bao,Weidi Kong,Xiaonan Hou,Rupeng Zhu 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.8
To examine the influence of the groove shape of a friction disk on the transient temperature field in the engagement process of aviation wet friction clutch, a transient thermal analysis model of friction clutch was established, and the application method of thermal boundary conditions, including the calculation of convective heat transfer coefficient and heat flux density, was studied. The transient heat loading process was established by the partition method of friction disk and steel disk. The temperature characteristics of wet clutch friction pairs with different groove shapes during the engagement process were analyzed and tested on an SAE#2 testing machine. Results show that when the clutch is engaged, the temperature of the friction pair composed of two-way parallel groove, three-way parallel groove, and waffle groove friction disk increases continuously at first and finally decreases slightly; the highest temperature of the friction disk and steel disk is at the ring near the outer ring of the friction disk;and the waffle groove friction disk is more suitable for aviation wet friction clutch.