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- 저자 : Xiangtao Chen (검색결과 2 건)
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Molecular Cloning, Characterization, and Expression Analysis of Chicken Δ-6 Desaturase
Kang, Xiangtao,Bai, Yichun,Sun, Guirong,Huang, Yanqun,Chen, Qixin,Han, Ruili,Li, Guoxi,Li, Fadi Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.1
Long-chain polyunsaturated fatty acids (LC-PUFA) promote the development of brain and vision of the fetus, relieve inflammation, inhibit oral dysplasia of rumor cell, decrease the incidence of cardiovascular disease and regulate arrhythmia. ${\Delta}-6$ desaturase is the rate-limited enzyme in the desaturation process. This study reports the cloning, characterization and tissue expression of a ${\Delta}-6$ desaturase gene in the chicken. PCR primers were designed based on the predicted sequence of chicken ${\Delta}-6$ desaturase (accession number: XM421053) and used to isolate a cDNA fragment of 1,323 bp from chicken liver. Based on the 1,323 bp fragment an EST (BI390105) was obtained by BLAST. The EST and 5'nd of the 1,323 bp fragment were partially overlapped. Gene specific primers derived from the EST were used for amplification of the 5'nd. Another gene-specific primer derived from the 1,323 bp fragment was used for amplification of the 3'nd by 3'ACE. Then the three overlapping cDNA sequences obtained were assembled with DNAMAN software and a full-length ${\Delta}-6$ desaturase of 2,153 bp was obtained. The full-length cDNA contained an ORF of 1,335 bp with a 5'ntranslated region of 147 nucleotides followed by an ATG initiation codon. Stop codon TGA was at position 1,481-1,483 bp. The deduced amino acids shared an homology above 77% with bovine, mice, orangutan, rat and human. The protein sequence had three histidine-rich regions HDFGH (HisI region), HFQHH (HisII region) and HH (HisIII region), a cytochrome $b_{5}$-like domain containing a heme-binding motif and two transmembrane domains. Sequence analysis of the chicken genomic DNA revealed that the coding sequence of chicken ${\Delta}-6$ desaturase included 12 exons and 11 introns. Semi-quantitative RT-PCR showed that the ${\Delta}-6$ desaturase expression levels were in turn liver, spleen, pancreas, lung, breast muscle, heart, and abdominal fat. The expression of ${\Delta}-6$ desaturase in liver was significantly higher than that in breast muscle (p<0.01). The expression of ${\Delta}-6$ desaturase in lung was significantly higher than that in abdominal fat (p<0.01). This is the first clone of chicken ${\Delta}-6$ desaturase.
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Predictor Variables’ Influence on Classification Outcome in Insurance Fraud Detection
Saliu Adam Muhammad,Xiangtao Chen,Liao Bo 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.5
In fraud detection paradigms, the role of predictor variables cannot be overemphasized particularly when analytical tools – such as statistical, machine learning and artificial intelligent tools are employed. These variables or attributes are used to organize records of data in database tables. The combination of the values of these attributes usually affects which class of a target variable a record or an observation would belong. In this paper, we propose an algorithm and employ spreadsheet ‘count’ ‘count if’ and ‘filtering’ functionalities (techniques) to take toll on how the individual attribute may affect the prediction of the class of an observation in an insurance dataset of 5000 observations. The analysis showed that indeed, the individual predictor attribute affects the outcome of the target variable (legal or fraudulent) differently.
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