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        Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar beet

        YingWu Shi,Chun Li,HongMei Yang,Tao Zhang,Yan Gao,Min Chu,Jun Zeng,Qing Lin,OuTiKuEr,YuGuo Li,Xiangdong Huo,Kai Lou 한국미생물학회 2017 The journal of microbiology Vol.55 No.4

        This study details the introduction of a gfp marker into anendophytic bacterial strain (Achromobacter marplatensisstrain 17, isolated from sugar beet) to monitor its colonizationof sugar beet (Beta. vulgaris L.). Stability of the plasmidencoding the gfp was confirmed in vitro for at least 72 h ofbacterial growth and after the colonization of tissues, undernonselective conditions. The colonization was observed usingfluorescence microscopy and enumeration of culturableendophytes in inoculated sugar beet plants that grew for 10or 20 days. gfp-Expressing strains were re-isolated from theinner tissues of surface-sterilized roots and stems of inoculatedplants, and the survival of the Achromobacter marplatensis17:gfp strain in plants 20 days after inoculation, evenin the absence of selective pressure, suggests that it is goodcolonizer. These results also suggest that this strain could bea useful tool for the delivery of enzymes or other proteinsinto plants. In addition, the study highlights that sugar beetplants can be used effectively for detailed in vitro studies onthe interactions between A. marplatensis strain 17 and its host,particularly if a gfp-tagged strain of the pathogen is used.

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