http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Xianbin Liu (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Taikui Zhang,Cuiyu Liu,Xianbin Huang,Hanyao Zhang,Zhaohe Yuan 한국식물학회 2019 Journal of Plant Biology Vol.62 No.1
Regulatory and developmental genes are retainedfollowing whole-genome duplication (WGD) events, and,thus, are central to elucidating the evolution of the gene familysubsequent to WGDs. Among these genes, the CYP75 genefamily is a key member of the biggest enzyme superfamilyin land-plant lineages. Although the molecular genetics ofthe biological progress involved with CYP75 genes havebeen partly elucidated, the evolution after WGDs in landplantlineages are still largely unknown. Here, we identifiedCYP75 orthologues in pomegranate (Punica granatum) andother twenty-five representative species to explore the geneevolution under WGD shaping on a broad evolutionary scale. Phylogenomic analyses identified genome-wide CYP75candidates and suggested that a recent duplication of theCYP75 genes in seed plants occurred prior to the split ofgymnosperms and angiosperms approximately 400 millionyears ago. Molecular evolution analyses revealed that CYP75gene lineages evolved under a different purifying selectionpressure, and slight relaxations occurred in the recentduplication groups in gymnosperms and angiosperms. Thesyntenic analyses showed that WGDs together with segmentalduplications contributed to the CYP75 gene evolution inpomegranate. RT-PCR, qRT-PCR and RNA-Seq verificationsuggested that pomegranate CYP75 genes evolved throughexon fusion and had a fruit-specific expression pattern. Neo- orsub-functionalization is the main fate of CYP75 genesfollowing duplication. The expression pattern of homologouscopies of CYP75 in pomegranate supports the CYP75 familyevolution contributing to species reproduction that showy fruitcolours attracted birds and other animals to spread seeds. Integration of the above analyses generated a putativeevolutionary scenario of the CYP75 family in land plants. Our data provided a potential reference model to furtherelucidate the evolution of the regulatory and developmentalgene families after WGDs.
-
-
Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3
Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
