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      • Random dynamic analysis for simplified vehicle model based on explicit time-domain method

        Huan Huang,Yuyu Li,Wenxiong Li,Guihe Tang Techno-Press 2023 Coupled systems mechanics Vol.12 No.1

        On the basis of the explicit time-domain method, an investigation is performed on the influence of the rotational stiffness and rotational damping of the vehicle body and front-rear bogies on the dynamic responses of the vehicle-bridge coupled systems. The equation of motion for the vehicle subsystem is derived employing rigid dynamical theories without considering the rotational stiffness and rotational damping of the vehicle body, as well as the front-rear bogies. The explicit expressions for the dynamic responses of the vehicle and bridge subsystems to contact forces are generated utilizing the explicit time-domain method. Due to the compact wheel-rail model, which reflects the compatibility requirement of the two subsystems, the explicit expression of the evolutionary statistical moment for the contact forces may be performed with relative ease. Then, the evolutionary statistical moments for the respective responses of the two subsystems can be determined. The numerical results indicate that the simplification of vehicle model has little effect on the responses of the bridge subsystem and the vehicle body, except for the responses of the rotational degrees of freedom for the vehicle subsystem, regardless of whether deterministic or random analyses are performed.

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        Interaction of Pseudostellaria heterophylla with Quorum Sensing and Quorum Quenching Bacteria Mediated by Root Exudates in a Consecutive Monoculture System<sup>s</sup>

        ( Liaoyuan Zhang ),( Zewang Guo ),( Huifang Gao ),( Xiaoqian Peng ),( Yongyu Li ),( Shujing Sun ),( Jung-kul Lee ),( Wenxiong Lin ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

        Many plant-pathogenic bacteria are dependent on quorum sensing (QS) to evoke disease. In this study, the population of QS and quorum quenching (QQ) bacteria was analyzed in a consecutive monoculture system of Pseudostellaria heterophylla. The isolated QS strains were identified as Serratia marcescens with SwrIR-type QS system and exhibited a significant increase over the years of monoculture. Only one QQ strain was isolated from newly planted soil sample and was identified as Bacillus thuringiensis, which secreted lactonase to degrade QS signal molecules. Inoculation of S. marcescens to P. heterophylla root could rapidly cause wilt disease, which was alleviated by B. thuringiensis. Furthermore, the expression of lactonase encoded by the aiiA gene in S. marcescens resulted in reduction of its pathogenicity, implying that the toxic effect of S. marcescens on the seedlings was QS-regulated. Meanwhile, excess lactonase in S. marcescens led to reduction in antibacterial substances, exoenzymes, and swarming motility, which might contribute to pathogensis on the seedlings. Root exudates and root tuber extracts of P. heterophylla significantly promoted the growth of S. marcescens, whereas a slight increase of B. thuringiensis was observed in both samples. These results demonstrated that QS-regulated behaviors in S. marcescens mediated by root exudates played an important role in replanting diseases of P. heterophylla.

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