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Yuan Gao,Yutong Shi,Na Miao,Wenxin Xing,Cholil Yun,Shengfang Wang,Wenjie Wang,Huimei Wang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-
In this study, a green and efficient ultrasound-assisted enzymatic extraction (UAEE) method was establishedfor total polyphenols (TPP) extraction from E. nigrum aerial parts. Fractional Factorial design (FFD)was initially implemented for the selection of significant parameters affecting TPP yield and then Box-Behnken design-response surface methodology (BBD-RSM) was employed to optimize the parameters. The optimum extraction conditions for UAEE were as follows: incubation temperature of 38.39 ℃, incubationtime of 3.39 h, tannase of 386.53 U/g of sample (U/g), and cellulase of 224.42 U/g. Under the optimumconditions, the TPP yield was 52.17 ± 0.39 mg/g, which was 1.62 and 1.73 times those of themethods of ultrasound-assisted extraction (UAE) and enzyme-assisted extraction (EAE), respectively. The crude extract was purified by HPD-600 microporous resin, and the purity was improved from8.11% to 22.56%. In addition, the results of antioxidant and antibacterial activity assays showed thatthe purified extract has stronger bioactivities in contrast with the crude extract. This study indicated thatUAEE was an effective technique for the extraction of TPP from the aerial parts of E. nigrum, and TPPextracted by this extraction method could be applied in pharmaceuticals, cosmetics, and food industries.