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Hongwei Li,Wenxiang Huang,Long Xu,Xiaosi Zhou,Hongxia Liu,Zhaobang Cheng 한국식물학회 2016 Journal of Plant Biology Vol.59 No.5
Cucumber green mottle mosaic virus (CGMMV) is a major limiting factor in the production of cucumber plants worldwide. In the present study, we use plant growth-promoting rhizobacteria (PGPR) to control this virus effectively. Stenotrophomonas maltophilia HW2 was isolated from healthy cucumber root, exhibited a good biocontrol efficacy against CGMMV. Here, it is documented that 20 d after virus inoculation, the biocontrol efficacy of HW2 reached 52.61%. HW2 can effectively colonize in cucumber rhizosphere, and also promoted cucumber plants growth. We also examined the effect of HW2 on viral replication and its mechanism. Compared with the control, HW2 pre-treated plants could delay virus replication for more than 3 d and inhibit viral protein genes (CP, MP, Rep) expression in the cucumber leaf. The expression of antioxidant enzyme genes (SOD and CAT) and defense-related genes (PR1 and PR5) were quickly induced by HW2. These results suggest that HW2 induced plant defense responses to CGMMV by increasing the expression of defense response genes. We report for the first time that Stenotrophomonas maltophilia improved cucumber resistance against CGMMV, which highlights the applying of PGPR on controlling of virus diseases.
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Zongping Chen,Xiang Liu,Wenxiang Zhou 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.27 No.4
This paper presents experimental results on the residual bond-slip behavior of high strength concrete-filled square steel tube (HSCFST) after elevated temperatures. Three parameters were considered in this test: (a) temperature (i.e., 20°C, 200°C, 400°C, 600°C, 800°C); (b) concrete strength (i.e., C60, C70, C80); (c) anchorage length (i.e., 250 mm, 400 mm). A total of 17 HSCFST specimens were designed for push-out test after elevated temperatures. The load-slip curves at the loading end and free end were obtained, in addition, the distribution of steel tube strain and the bond stress along the anchorage length were analyzed. Test results show that the shape of load-slip curves at loading ends and free ends are similar. With the temperature constantly increasing, the bond strength of HSCFST increases first and then decreases; furthermore, the bond strength of HSCFCT proportionally increases with the anchoring length growing. Additionally, the higher the temperature is, the smaller and lower the bond damage develops. The energy dissipation capacity enhances with the concrete strength rasing, while, decreases with the temperature growing. What is more, the strain and stress of steel tubes are exponentially distributed, and decrease from the free end to loading end. According to experimental findings, constitutive formula of the bond slip of HSCFST experienced elevated temperatures is proposed, which fills well with test data.
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