http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Wenbo Chai (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Functional analysis of the HD-Zip I gene ZmHDZ1 in ABA-mediated salt tolerance in rice
Qianqian Wang,Kangyong Zha,Wenbo Chai, Yu Wang,Bin Liu,Haiyang Jiang,Beijiu Cheng,Yang Zhao 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2
The homeodomain-leucine zipper I (HD-Zip I) transcription factors play crucial roles in the regulation of abiotic stress responses, whereas, up to now, most of them are still functionally unknown. In present study, we identified a maize HD-Zip I gene, designing as ZmHDZ1, and investigated its potential roles in salt stress response. The qRT-PCR results showed that expression of ZmHDZ1 was induced by ABA and salt stress. Transactivation assay and transient expression analyses indicated that ZmHDZ1 protein was located in the nucleus in tobacco leaf cells and had the transactivation activity in yeast. Over-expression of ZmHDZ1 in rice reduced tolerance to salt stress, thus, led to greater accumulation of malondialdehyde (MDA) as well as higher level of relative electrolyte leakage (REL) compared to WT plants. In addition, the transgenic seedlings also increased sensitivity to exogenous ABA. Furthermore, the expression levels of four salt responsive genes were different between transgenic and WT rice under normal or NaCl treatment. These results suggested that ZmHDZ1 function as a negative regulator in response to salt stress through the ABAmediated signal transduction pathways.
-
Yang Zhao,Xiaojian Peng,Wenbo Chai,Yingquan Tan,Qing Dong,Haiyang Jiang,Beijiu Cheng 한국유전학회 2017 Genes & Genomics Vol.39 No.1
Integrated networks of gene expression, hormonal signaling and metabolite sensing regulate phyllotaxy pattern development. In this study, we characterized differentially expressed genes (DEGs) between maize plants with alternate and opposite phyllotaxies. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that 2432 DEGs were involved in biological processes, molecular functions, cellular components and many pathways. Importantly, we identified 19 DEGs related to plant hormone signal transduction. Additionally, we identified four main alternative splicing types: skipped exons, retained introns, alternative 50-splice sites, and alternative 30-splice sites, which exhibited different characteristics in the alternate and opposite phyllotaxy libraries. The reliability of the sequencing data was verified through using quantitative real-time reverse transcription PCR analysis of the 19 genes: 15 were validated to play a role in phytohormone signal transduction pathways. Taken together, our data provide new insight into the mechanisms of phyllotaxy pattern development, and will increase our understanding of how relative changes in gene expression determine alternate/opposite phyllotaxy in maize.
-
PWR core calculation based on pin-cell homogenization in three-dimensional pin-by-pin geometry
Zhang Bin,Li Yunzhao,Wu Hongchun,Zhao Wenbo,Fang Chao,Gong Zhaohu,Li Qing,Chai Xiaoming,Yu Junchong 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.6
For the pressurized water reactor two-step calculation, the traditional assembly homogenization and two-group neutron diffusion calculation have been widely used. When it comes to the core pin-by-pin simulation, many models and techniques are different and unsettled. In this paper, the homogenization methods based on the pin discontinuity factors and super homogenization factors are used to get the pin-cell homogenized parameters. The heterogeneous leakage model is applied to modify the infinite flux spectrum of the single assembly with reflective boundary condition and to determine the diffusion coefficients for the SP3 solver which is used in the core simulation. To reduce the environment effect of the single-assembly reflective boundary condition, the online method for the SPH factors updating is applied in this paper, and the functionalization of SPH factors based on the least-squares method will be pre-made alone with the table of the group constants. The fitting function will be used to update the thermal-group SPH factors with a whole-core pin-by-pin homogeneous solution online. The three-dimensional Watts Bar Nuclear Unit 1 (WBN1) problem was utilized to test the performance of pin-by-pin calculation. And numerical results have demonstrated that PWR pin-by-pin core calculation has more accurate results compared with the traditional assembly-homogenization scheme.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
