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Min Jiang,Changling Zhao,Mingfu Zhao,Yongzhong Li,Guosong Wen 한국식물학회 2020 Journal of Plant Biology Vol.63 No.2
Calcium signals play critical functions in regulating diverse arrays of plant growth and development and mediating a variety of biotic and abiotic stress responses as a second messenger. Calcineurin B-like (CBL) proteins were involved with plantspecifc Ca2+signaling as calcium sensors. In this work, we retrieved 152 CBL gene members from 15 diferent grass species, surveyed their phylogenetic relationships and sequence features and also performed expression patterns and functional analyses of rice CBLs. Phylogenetic analysis indicated that grass CBLs fall into four diferent groups (Group A–D). Sequence analysis showed that CBL proteins harboring four conserved calcium-binding EF-hand have key amino acid residues Asp and Glu which had relatively high proportion in the average abundance. Molecular evolutionary analyses revealed that group A, B and C CBLs in their evolution process sufered the purifying selection, while group D CBLs were subjected to positive selection. Moreover, expression analyses showed signifcant divergent expression patterns of OsCBLs in various organs and under diferent hormones and abiotic stresses. Furthermore, tolerance analysis revealed that OsCBL3 and OsCBL8 overexpression transgenic rice seedlings improved salt tolerance and OsCBL5, OsCBL6 and OsCBL7 positively regulated drought stress. In general, the domain and base sequence of the CBL gene family is highly conserved in grasses. OsCBL genes had specifc gene expression profles and function in diferent stresses.
Ma Xiaowen,Gu Jinbao,Luo Qingwen,Wen Mingfu,Li Hua,Wang Zhen-Yu 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.1
β-Aminobutyric acid (BABA) and γ-aminobutyrate (GABA) had a positive role in alleviating the damage when plants were exposed to abiotic and biotic stresses. However, it is not known whether exogenous of GABA and BABA could improve the tolerance of cassava plants, and their synergistic benefits. In the current study, we found that the cassava seedling improved the tolerance by exogenous of BABA and GABA under salt and drought stress condition, as reflected by reducing the accu- mulation of hydrogen peroxide and increasing the activities of antioxidant enzymes. Meanwhile, the expression of genes encoding antioxidant enzymes was significantly unregulated by salt or drought stress after application of BABA and GABA, separately. Moreover, the transcript levels of DREB2A and SOS2 were significantly unregulated by salt after application of BABA and GABA, separately, and expression of DREB2A, NCED3, and CBF3 was significantly unregulated by drought stress after application of BABA and GABA, separately. Interestingly, there was a synergistic benefit on improving the plant stress tolerance by combining the application of BABA and GABA. Therefore, our study provides new insights into maintaining the high yield by combining use of the chemical compounds in cassava which is relatively hard to be genetically modified.