http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : W.J. Banz (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
In Vitro Effects of Soy Phytoestrogens on Rat L6 Skeletal Muscle Cells
K.L. Jones,J. Harty,M.J. Roeder,T.A. Winters,W.J. Banz 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.3
Soy isoflavones display estrogenic activity in humans and animals, and thus are referred to as phytoestrogens.This study was performed to observe the effects of the soy isoflavones genistein, daidzein, and glycitein on cell cultures ofrat skeletal muscles. [3H]Thymidine incorporation was used to determine cell proliferation, while protein synthesis and degra-dation were determined by tracking radiolabeled leucine. For the proliferation studies, insulin, estradiol, genistein, daidzein,or glycitein was supplemented at 0, 0.04, 0.08, 0.16, 0.31, 0.63, 1.25, 2.5, 5, 10, or 20 .M, respectively, or in combinationswith final concentrations of 0, 0.1, 1, or 10 .M. Genistein reacted most similarly to estradiol, inhibiting proliferation at . 1.M (P. .001). A combination of phytoestrogens resulted in significant inhibition of cell proliferation, but not to the extentobserved with genistein alone. For the protein synthesis and degradation experiments, treatments of 0.1 .M dexamethasoneor 1 .M concentrations of insulin, genistein, daidzein, or glycitein were used. Phytoestrogens did not inhibit or stimulate pro-tein degradation or synthesis (P. .05). A one-tailed univariate analysis of variance revealed a trend (P. .1) in protein stim-ulation with genistein and glycitein treatments. These results suggest that the tyrosine kinase inhibiting activity of genisteinmay be affecting phosphorylation of the mitosis-promoting factor, preventing the advancement of the mitotic cell cycle. Inaddition, at higher total combined concentrations, daidzein and glycitein may be able to outcompete genistein for receptorsites. These results suggest that soy isoflavones in the diet may potentially modulate normal growth and development in hu-mans and animals that ingest soy-based products.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
