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Biogenic synthesis of gold nanoparticles from Halymenia dilatata for pharmaceutical applications: Antioxidant, anti-cancer and antibacterial activities

Vinosha, Manoharan,Palanisamy, Subramanian,Muthukrishnan, Ramasamy,Selvam, Samayanan,Kannapiran, Ethiraj,You, SangGuan,Prabhu, Narayanasamy Marimuthu Elsevier 2019 PROCESS BIOCHEMISTRY Vol.85 No.-
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Abstract Biogenic synthesis of metal nanoparticles using seaweeds is more biocompatible and more valuable for biomedical applications. In this study, gold nanoparticles were synthesized from the aqueous extract of Halymenia dilatata (Hd-AuNPs) and characterized by various spectral and microscopic techniques. The Hd-AuNPs exhibit a surface plasmon resonance peak at 529 nm. FT-IR results confirm the presence of polysaccharides and pigments in the extract, which reduce Au3+ to Au0. Microscopic analysis confirmed that Hd-AuNPs were triangular and spherical in shape with an average size of 16 nm. In terms of potential antioxidant properties, DPPH radical scavenging (13.7 ± 0.40 to 58.7 ± 0.85%), reducing power (16.3 ± 0.63 to 62.4 ± 0.60%) and total antioxidant (12.4 ± 0.50 to 56.6 ± 0.74%) activities were reasonably high in Hd-AuNPs compared with the extract alone. In the cytotoxicity assay, the IC50 of Hd-AuNPs was 22.62 μg/ml against HT-29 cancer cells. Similarly, in the antibacterial assay, the highest zone of inhibition (21 mm) was recorded at 100 μg/ml against Aeromonas hydrophila and the activity was proportionate to the concentration of Hd-AuNPs. In addition, the biosynthesis of Hd-AuNPs was non-toxic to zebrafish embryo. Overall, synthesized Hd-AuNPs exhibit commendable antioxidant, anti-cancer and antibacterial activities. Highlights <UL> <LI> Biogenic synthesis of gold nanoparticles using the aqueous extract of H.dilatata </LI> <LI> The synthesized Hd-AuNPs were confirmed by spectral and microscopic examination. </LI> <LI> Antioxidant, Anti-cancer and Antibacterial properties of Hd-AuNPs were studied. </LI> <LI> First report on the biological activities of GNPs synthesized from H. dilatata. </LI> </UL> Graphical abstract [DISPLAY OMISSION]
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Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum

Palanisamy, Subramanian,Vinosha, Manoharan,Rajasekar, Periyannan,Anjali, Ravichandran,Sathiyaraj, Ganesan,Marudhupandi, Thangapandi,Selvam, Samayanan,Prabhu, Narayanasamy Marimuthu,You, SangGuan Elsevier 2019 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.125 No.-
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Abstract In this study therapeutic potential of fucoidan fraction-2 (Fu-F2) isolated from Sargassum polycystum was evaluated for the development of antibacterial agent against the human and animal pathogenic bacteria by in vitro and in vivo analysis. The Fu-F2 contained 51.12 ± 0.86% of total sugar and 20.41 ± 0.91% of sulfate. The structural characterization of Fu-F2 was performed by HPLC, FTIR and NMR analysis and reported in our earlier study. The in vitro antibacterial assays such as MIC, MBC, killing kinetics, disk diffusion, protein leakage, ROS and confocal laser scanning microscopy demonstrate that Fu-F2 possesses the highest antibacterial activity against the tested pathogens. Among the tested pathogens, the highest antibacterial activity (21 ± 1.0 mm) was recorded at the concentration of 50 μg/ml against Pseudomonas aeruginosa and the lowest activity (16 ± 0.53 mm) was registered against Staphylococcus aureus. In the in vivo analysis, the pre-treatment group with Fu-F2 at the concentration of 15 mg/0.1 kg through feed exhibited the highest survival (83.4%) and antioxidant activities (p < 0.05) than the fish infected with pathogen. Thus, the present findings suggest that the Fu-F2 of S. polycystum encompasses significant antibacterial properties and that can be used as a therapeutic agent for controlling the bacterial disease. Highlights <UL> <LI> The fucoidan fraction-2 (Fu-F2) was successfully isolated from Sargassum polycystum. </LI> <LI> The antibacterial activity of Fu-F2 was tested by various in vitro and in vivo assays. </LI> <LI> The Fu-F2 shows higher antibacterial activity against P. aeruginosa than the other bacteria. </LI> <LI> The in vivo studies confirmed that Fu-F2 treated zebrafish shows excellent antioxidant properties and improved the histological architecture. </LI> </UL>
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Synthesis of Oldenlandia umbellata stabilized silver nanoparticles and their antioxidant effect, antibacterial activity, and bio-compatibility using human lung fibroblast cell line WI-38

Subramanian, Palanisamy,Ravichandran, Anjali,Manoharan, Vinosha,Muthukaruppan, Reka,Somasundaram, Selvakumar,Pandi, Boomi,Krishnan, Anand,Marimuthu, Prabhu Narayanasamy,Somasundaram, Selliah Swamy Nat Elsevier 2019 Process biochemistry Vol.86 No.-
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Abstract In the past few years, green synthesized silver nanoparticles (AgNPs) are one of predominantly used materials in therapeutics and clinical practices. In this study, AgNPs were synthesized from aqueous extract of Oldenlandia umbellata and investigated their pharmaceutical properties. UV–vis spectroscopy of O. umbellata mediated silver nanoparticles (AgNPs@OUa) showed a peak at 423 nm. Fourier transform infrared spectroscopy and phytochemical screening explained the contribution of biomolecules in AgNPs@OUa synthesis. Field emission scanning electron microscope and high-resolution transmission electron microscope examination revealed that small monodispersed spherical nanoparticles had an average size of 22.7 nm. Antioxidant assays were used to examine antioxidant properties of AgNPs@OUa in comparison with L-ascorbic acid (Vitamin C) as a standard antioxidant. AgNPs@OUa showed dose-dependent antioxidant activities. Antibacterial activities of AgNPs@OUa exhibited the highest zone of inhibition against Streptococcus mutans and Escherichia coli were 16.03 ± 0.35 mm and 19.76 ± 0.25 mm, respectively, at concentration of 1.0 mg L−1. Biocompatibility of AgNPs@OUa against human lung fibroblast (WI-38) cell line was also tested. Even though, the cells treated with the uppermost concentration (100 μg/mL) of AgNPs@OUa, it enhances the cell viability (∼90%), suggesting that AgNPs@OUa had no toxicity with excellent biocompatibility. Highlights <UL> <LI> First report on the bio synthesis of AgNPs using O. umbellata aqueous extract. </LI> <LI> The AgNPs@OUa were characterized by spectral and microscopy analysis. </LI> <LI> AgNPs@OUa were act as a significant antioxidant and antibacterial activities. </LI> <LI> AgNPs@OUa showed excellent biocompatibility against WI-38 cell lines. </LI> </UL> Graphical abstract [DISPLAY OMISSION]

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