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U. Yong(용의중),D. Kim(김동환),H. Kim(김호중),D. G. Hwang(황동규),S. Cho(조성건),H. Nam(남효영),S. Kim(김세진),T. Y. Kim(김태영),U. Jeong(정운룡),K. Kim(김기훈),W. K. Chung(정완균),W. H. Yeo(여운홍),J. Jang(장진아) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월
Over the years, engineered heart tissue (EHT), composed of cardiac cells and a hydrogel, has been considered as a promising in-vitro cardiac model in that it can reproduce the physiological contractions of an actual animal heart. In particular, the contractile force of EHT is one of the representative factors to evaluate drug-induced cardiotoxicity that is a major cause of the withdrawal of drug development. Although there have been a lot of methods to monitor the contractile force of the EHT, most of them are based on optical readout systems that have to process a huge amount of image data. Recently, a strain gauge-based microphysiological system was developed to monitor the contractile force of a laminar cardiac tissue, which can acquire real-time data with a relatively small amount of data. However, the system can monitor only few layers of cardiomyocytes, which are physiologically less relevant environment compared to EHT. Here, we developed a hybrid bioprinted tissue platform, consisting of six bipillar-grafted strain gauges (BPSGs) and one wireless device, that enables online monitoring of the contractile forces from 6 different EHTs in real time during culturing. Furthermore, we confirmed that our system can detect the effects of commercially available drugs on EHTs.
H. Han(한호현),Y. Park(박예진),Y. Choi(최유미),U. Yong(용의중),B. Kang(강병민),W. Shin(신우정),S. Min(민소연),H. J. Kim(김현중),J. Jang(장진아) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월
Intestinal disease is a global health problem affecting millions of people. To investigate therapeutic compounds and unveil the mechanisms of the diseases, numerous in vitro intestine models have been developed as alternatives to animal surrogates. Intestinal organoids have shown that they can emulate major human physiology, but it is difficult to control their growth and maturation. On the other hand, microphysiological systems (MPSs) have been enabled to recapitulate intestinal tissues in vitro and its ecosystem with commensal microbiomes, their geometrical complexity is limited to 2D or 2.5D architectures. For this reason, 3D bioprinting has gained growing interest since it can be used to mimic 3D complex physiology such as cell-cell or cell-matrix interaction. Here, we bioprinted a tubular intestine model using a colon-derived decellularized extracellular matrix (Colon dECM). We observed that Colon dECM promotes spontaneous maturation of intestinal epithelial cells, especially its enteroendocrine functions. Furthermore, the 3D bioprinted tubular model with a hollow lumen showed cellular maturation and self-organization demonstrating distinctive tissue morphogenesis. We envisage this advanced in vitro intestine model as a platform to study intestinal morphogenesis, disease, and treatments.