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Tianxiu Zhong,Huiming Zeng,Liebao Han,Lijuan Zhang,Shanshan Sun 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.5
Drought resistance is increased in plants by theabsence of the hormone gibberellic acid (GA) or by a lackof GA sensitivity. We studied the effects of tissue-specificreduction in GA levels on drought tolerance, on recoveryfrom drought stress, and on primary and secondary growthusing transgenic tobacco plants expressing the GA-inactivatinggene PtGA2ox1 (GA 2-oxidase) specifically inleaves, stems, or roots. Localized reduction of bioactiveGA1 levels was achieved by tissue-specific expression ofthe PtGA2ox1 gene in leaves using the rbcs promoter (LDplants), in roots using the TobRB7 promoter (RD plants),and in stems using the LMX5 promoter (SD plants). Inresponse to drought stress, all transgenic tobacco plantsexhibited reduced primary and secondary growth andincreased drought tolerance with a corresponding reductionin malondialdehyde levels, higher relative water content,increased proline and sugar content, and elevated peroxidase,superoxide dismutase, and catalase activities relativeto wild-type plants. The highest level of drought toleranceand the most rapid recovery from stress was achieved bylocalized reduction of GA1 in the roots of the RD transgenicplants. In addition, although the total bioactive GA1content in RD and LD plants was essentially identical, theheights of LD plants were significantly greater and droughttolerance was significantly less than in RD plants. It ispossible that the site of gibberellin-related gene expressionplays an important role in the balance between growth anddrought tolerance.