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RISS 인기검색어
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- 저자 : Thun Vathany (검색결과 4 건)
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Large-Sized Cormlet Induction and BYMV Removal through Tissue Culture in Gladiolus
Vathany Thun,Mi Soon Byun,Dae Hoe Goo,Kiu Weon Kim 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.5
Gladiolus ‘Adlib Scarlet’, ‘May Queen’, ‘Pacific Pink’, ‘Sharone’ and ‘White Race’ were used as plant materials in order to obtain large-sized cormlets from a shoot base in vitro for enhancing survival rate after soil transplanting, and large cormel tips in size 1 ㎣ were used as explants and subcultured for five to six times to remove the bean yellow mosaic potyvirus (BYMV) for enhancing efficiency of tissue culture. Cormlets were the largest in 9% sucrose compared to glucose, fructose and glucose+fructose in all cultivars tested. The 1:2 NH₄?:NO₃? ratio in MS solid medium containing 9% sucrose was the best for enlarging cormlets in almost all cultivars; hence, decrease of NO₃? concentration in MS medium is needed. Plantlets without cormlets did not survive in greenhouse. Cormlets produced in solid medium were sprouted 100% and grown to vigorous plants in all cultivars. Survival rate was 30~85% by cultivars in cormlets obtained in liquid medium. Maintenance of the plantlets in MS solid medium is needed prior to their transfer to soil for enhancing survival rate. Though the cormlets were obtained from large-sized cormel tip, BYMV were removed through subculture for five to six times.
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Effect of In Vitro Culture Environments and Culture Methods on Cormlet Formation of Gladiolus
Vathany Thun,Dae-Hoe Goo,Min Hee Kim,Mi Soon Byun,Kiu Weon Kim 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.2
Five gladiolus cultivars 'Adlib Scarlet', 'May Queen', 'Pacific Pink', 'Sharone' and 'White Race' were used in order to identify their specific culture environments and culture methods to obtain cormlets from a shoot base in vitro. There was a cultivar-by-medium-temperature interaction effect. The solid medium was appropriate only for cormlet formation of 'May Queen', while liquid medium was favored for the other four cultivars. The best culture enviroment for cormlets formation of 'Pacific Pink', 'Sharone' and 'White Race' was liquid medium treated at 20℃. Fresh weight of cormlets of 'Adlib Scarlet' was highest when grown in liquid medium treated at 25℃. There was no difference among the three temperatures for cormlet formation of 'May Queen' which grew best in solid medium. The suitable light intensity was 68 μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> PAR for 'May Queen', 'Sharone' and 'White Race', 34 μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> PAR for 'Adlib Scarlet' and 135 μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> PAR for 'Pacific Pink'. Increase in fresh weight was related to increased diameter regardless of different temperature, light intensity, media, or cultivar.
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Ouch Sreyneth,Thun Vathany,송영주,이응호 한국국제농업개발학회 2023 韓國國際農業開發學會誌 Vol.35 No.4
In 2006, Cambodian Agriculture Research and Development Institute (CARDI) bred a high-quality tomato ‘Neang Pich’ variety. Since ‘Neang Pich’ is susceptible to Sclerotium rolfsii (S. rolfsii), it is severely affected the production major tomato growing regions. This study was conducted to evaluate the effect of seven S. rolfsii tolerant rootstocks on growth and yield in graft cultivation of ‘Neang Pich’ tomato. ‘Neang Pich’ seedlings were used as scion and non-grafted control. Six eggplant genotypes (‘2017053’, ‘2017062’, ‘17CJVC2’, ‘No. 80’, ‘VI041979A’, ‘VI041996’) and a commercial tomato variety (‘Hulk’) were used as rootstock. The grafted and non-grafted tomatoes were grown in a plastic greenhouse and open field. The survival rate of plants did not differ between plastic greenhouse (80.2%) and open field (79.5%). The top and root fresh weight of plants grown in a plastic greenhouse increased by 77% and 11% compared to the open field and the dry weight increased by 48% and 10%, respectively. The top (309 g) and root (18.9 g) fresh weight, and the top (90.5 g) and root (6.39 g) dry weight depending on rootstock were the highest in plants grafted onto ‘2017062’. The yield of tomatoes in a plastic greenhouse (19.5 MT/ha) was 65% higher than that of open field (11.8 MT/ha). The yield of tomatoes depending on rootstock were the highest in ‘2017062’ (17.8 MT/ha). The effect of cultivation practice and grafting on pH and sugar content (o Brix) of the fruit was non-significant difference (p≤0.05). The scion diameter, top fresh weight, fruit weight and yield (MT/ha) were significant difference (p0.05) in the interaction effect between tomato cultivation practices (plastic greenhouse and open field) and rootstock.
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