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- 저자 : Terrence M. Vance (검색결과 2 건)
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Anthocyanin-Rich Blackcurrant Extract Attenuates Ovariectomy-Induced Bone Loss in Mice
Xi Zheng,Sewhan Mun,이상길,Terrence M. Vance,Patrice Hubert,Sung I. Koo,Sun-Kyeong Lee,Ock K. Chun 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.4
Although several animal and cell studies have indicated that blackcurrant anthocyanins exert antioxidative andanti-inflammatory properties, which could potentially improve bone mass, the effect of blackcurrant on bone health has notbeen reported yet. Thus, this study was aimed to evaluate the effect of blackcurrant anthocyanins on bone mass in an estrogendeficiency mouse model. Fourteen-week-old C57BL/6J mice (n = 54) were ovariectomized or sham operated. The ovariectomizedmice were divided into two groups, basal diet (OVX) or basal diet with 1% anthocyanin-rich blackcurrant extract(OVX+BC), and sacrificed at 4, 8, and 12 weeks. Femoral bone mineral density (BMD) and trabecular bone volume by dualenergyX-ray absorptiometry and micro-computed tomography, respectively, and serum bone markers were measured. Ovariectomy significantly reduced BMD and trabecular bone volume at all time points (P < .05). Blackcurrant supplementationattenuated ovariectomy-induced bone loss measured by BMD and trabecular bone volume at 8 weeks (P = .055 andP = .057) and the effect was more pronounced at 12 weeks (P = .053 and P < .05). Ovariectomy and blackcurrant treatment didnot alter serum biomarkers of bone formation and resorption. Bone marrow cells extracted from OVX mice significantlyinduced osteoclast-like (OCL) cell formation compared with cells from sham controls (P < .05). Blackcurrant treatmentdecreased the number of TRAP(+) OCL compared with OVX mice at 8 and 12 weeks (P < .05). Furthermore, blackcurrantsupplementation reduced bone resorption activity when measured by resorption pit assay, compared with OVX group(P < .05). These results demonstrate that blackcurrant may be effective in mitigating osteoclast-induced postmenopausalbone loss.
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( Sang Gil Lee ),( Taoran Wang ),( Terrence M. Vance ),( Patrice Hurbert ),( Dae-ok Kim ),( Sung I. Koo ),( Ock K. Chun ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.2
Although several analytical methods for measuring total antioxidant capacity (TAC) have been applied to biological samples, there were often dissimilar results due to the different principles of methods applied. Thus, this study aimed to validate four conventional analytical methods for measuring plasma TAC, including the ABTS assay, DPPH assay, FRAP assay, and ORAC assay, by comparing with urinary 8-isoprostane concentration. In addition, TAC results were compared with antioxidant enzyme activities including superoxide dismutase (SOD) and glutathione peroxidase in erythrocyte, and catalase in plasma. Plasma TAC measure by ABTS assay was strongly correlated with the result by FRAP assay. Plasma TAC by FRAP and ORAC assays were negatively correlated with erythrocyte SOD activity. The agreement among the four TAC assay methods and 8-isoprostane was determined using 95% prediction limits of linear regression, expressed as the mean of 8-isoprostane ± 95% prediction limits. The ABTS method better agreed with 8-isoprostane than the other methods, demonstrating narrow prediction of limits. Furthermore, only plasma TAC determined by the ABTS assay was inversely correlated with urinary 8-isoprostane (r = -0.35, p < 0.05). In summary, the ABTS assay would be an appropriate method to measure overall plasma antioxidant capacity and predict the body`s antioxidant status.
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