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RISS 인기검색어
- 검색키워드
- 저자 : Tembhare, Dnyaneshwar Bapuji (검색결과 5 건)
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Genome-wide identification of auxin response factors (ARFs) in three different species of Arachis
Raul Bikash,Bhattacharjee Oindrila,Tembhare Kunal,Khanna Tanyya,Shaheen Tarannum,Sinharoy Senjuti,Bandyopadhyay Kaustav 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.2
The phytohormone auxin is involved in the regulation of plant growth, nutrient acquisition, and response to environmental stimuli. Auxin response factors (ARFs) are transcription factors containing B3 DNA binding domain. ARFs play central role in auxin response, using Aux/IAA proteins as partners. Arachis is a genus within the Dalbergioid clade of papilionoid legumes, which out-branched from other members of papilionoids. Cultivated peanut (Arachis hypogaea L.) is an allotetraploid formed by hybridization of two parental genotypes Arachis duranensis, and Arachis ipaensis merely 10,000 years ago. We have made a genome-wide inventory of all the ARFs present in tetraploid A. hypogaea, as well as in two diploid parental genotypes. Our data show that Arachis contains more ARFs per diploid genome (around 31), compared to other legumes (around 25). We further observed few ARF-like genes which are defective in important domains. Most of the ARFs in tetraploid Arachis are redundant, representing the A and B sub-genomes. Some of the ARFs show expression bias from either A or B sub-genome, while some of the pairs are expressed from both sub-genomes. Many ARFs do not express in any of the conditions for which we have expression data. Finally, few pairs show diferential spatio-temporal expression pattern from A and B sub-genomes, indicative of diversifcation of function. This is the frst efort to list all the ARFs from an allotetraploid legume. The list of ARFs in all three species of Arachis will help the scientifc community working to understand auxin regulation in crop legumes. The phytohormone auxin is involved in the regulation of plant growth, nutrient acquisition, and response to environmental stimuli. Auxin response factors (ARFs) are transcription factors containing B3 DNA binding domain. ARFs play central role in auxin response, using Aux/IAA proteins as partners. Arachis is a genus within the Dalbergioid clade of papilionoid legumes, which out-branched from other members of papilionoids. Cultivated peanut (Arachis hypogaea L.) is an allotetraploid formed by hybridization of two parental genotypes Arachis duranensis, and Arachis ipaensis merely 10,000 years ago. We have made a genome-wide inventory of all the ARFs present in tetraploid A. hypogaea, as well as in two diploid parental genotypes. Our data show that Arachis contains more ARFs per diploid genome (around 31), compared to other legumes (around 25). We further observed few ARF-like genes which are defective in important domains. Most of the ARFs in tetraploid Arachis are redundant, representing the A and B sub-genomes. Some of the ARFs show expression bias from either A or B sub-genome, while some of the pairs are expressed from both sub-genomes. Many ARFs do not express in any of the conditions for which we have expression data. Finally, few pairs show diferential spatio-temporal expression pattern from A and B sub-genomes, indicative of diversifcation of function. This is the frst efort to list all the ARFs from an allotetraploid legume. The list of ARFs in all three species of Arachis will help the scientifc community working to understand auxin regulation in crop legumes.
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Royal Jelly Protein and Lipid Composition in Apis cerana indica F.
Shinkhede, Milind Manohar,Tembhare, Dnyaneshwar Bapuji Korean Society of Sericultural Science 2009 International Journal of Industrial Entomology Vol.18 No.2
The histological and transmission electron microscopic studies revealed the synthesis activity predominantly in the hypopharyngeal glands of the nurse bees. The biochemical analysis of both, the hypopharyngeal gland extract and royal jelly elucidated unequivocally the proteins and lipids as the major constituents. Further the SDS-PAGE of hypopharyngeal gland extract showed about 17 protein bands, perhaps 14.10, 20.00, 29.00 and 43.00 kDa predominantly while that of royal jelly revealed only two protein bands of 29.00 and 43.00 kDa molecular weight suggesting them as the major royal jelly proteins (MRJP). The lipid profile of royal jelly consists of triglycerides, cholesterol, HDL, LDL and VLDL.
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Barsagade, Deepak Dewaji,Pankule, Sushama Dilip,Tembhare, Dnyaneshwar Bapuji Korean Society of Sericultural Science 2009 International Journal of Industrial Entomology Vol.18 No.2
The egg shell of the tropical tasar silkworm, Antheraea mylitta is formed from the substances secreted by the follicular epithelium during the late vitellogenic stage. TEM study reveals the inner travecular and outer lamellar layer of chorion. The travecular layer is composed of the innermost wax layer, inner and outer chorionic layer. The inner and outer chorionic layers are connected to each other by vertical pillers forming of cavities. The lamellar layer is perforated by the aeropyles. SEM study reveals the differentiation of an anterior surface of the egg shell into four zones-micropylar, edge, aeropyles crown and disc zone. In the mycosis infected eggs the aeropyles and egg-shell surface are fully packed with the hyphae of the fungus, Aspergillus sydowi blocking of plastron respiration and causing the death of developing embryo so that mycosis infected eggs become sterile.
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Royal Jelly Protein and Lipid Composition in Apis cerana indica F.
( Milind Manohar Shinkhede ),( Dnyaneshwar Bapuji Tembhare ) 한국잠사학회 2009 International Journal of Industrial Entomology Vol.18 No.2
The histological and transmission electron microscopic studies revealed the synthesis activity predominantly in the hypopharyngeal glands of the nurse bees. The biochemical analysis of both, the hypopharyngeal gland extract and royal jelly elucidated unequivocally the proteins and lipids as the major constituents. Further the SDS-PAGE of hypopharyngeal gland extract showed about 17 protein bands, perhaps 14.10, 20.00, 29.00 and 43.00 kDa predominantly while that of royal jelly revealed only two protein bands of 29.00 and 43.00 kDa molecular weight suggesting them as the major royal jelly proteins (MRJP). The lipid profile of royal jelly consists of triglycerides, cholesterol, HDL, LDL and VLDL.
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Deepak Dewaji Barsagade,Sushama Dilip Pankule,Dnyaneshwar Bapuji Tembhare 한국잠사학회 2009 International Journal of Industrial Entomology Vol.18 No.2
The egg shell of the tropical tasar silkworm, Antheraea mylitta is formed from the substances secreted by the follicular epithelium during the late vitellogenic stage. TEM study reveals the inner travecular and outer lamellar layer of chorion. The travecular layer is composed of the innermost wax layer, inner and outer chorionic layer. The inner and outer chorionic layers are connected to each other by vertical pillers forming of cavities. The lamellar layer is perforated by the aeropyles. SEM study reveals the differentiation of an anterior surface of the egg shell into four zones-micropylar, edge, aeropyles crown and disc zone. In the mycosis infected eggs the aeropyles and egg-shell surface are fully packed with the hyphae of the fungus, Aspergillus sydowi blocking of plastron respiration and causing the death of developing embryo so that mycosis infected eggs become sterile.
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