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- 저자 : Teixeira Lucas Soares (검색결과 2 건)
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A novel antimicrobial-containing nanocellulose scaffold for regenerative endodontics
Kichler Victoria,Teixeira Lucas Soares,Prado Maick Meneguzzo,Colla Guilherme,Schuldt Daniela Peressoni Vieira,Coelho Beatriz Serrato,Porto Luismar Marques,de Almeida Josiane 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.2
Objectives: The aim of this study was to evaluate bacterial nanocellulose (BNC) membranes incorporated with antimicrobial agents regarding cytotoxicity in fibroblasts of the periodontal ligament (PDLF), antimicrobial activity, and inhibition of multispecies biofilm formation. Materials and Methods: The tested BNC membranes were BNC + 1% clindamycin (BNC/CLI); BNC + 0.12% chlorhexidine (BNC/CHX); BNC + nitric oxide (BNC/NO); and conventional BNC (BNC; control). After PDLF culture, the BNC membranes were positioned in the wells and maintained for 24 hours. Cell viability was then evaluated using the MTS calorimetric test. Antimicrobial activity against Enterococcus faecalis, Actinomyces naeslundii, and Streptococcus sanguinis (S. sanguinis) was evaluated using the agar diffusion test. To assess the antibiofilm activity, BNC membranes were exposed for 24 hours to the mixed culture. After sonicating the BNC membranes to remove the remaining biofilm and plating the suspension on agar, the number of colony-forming units (CFU)/mL was determined. Data were analyzed by 1-way analysis of variance and the Tukey, Kruskal-Wallis, and Dunn tests (α = 5%). Results: PDLF metabolic activity after contact with BNC/CHX, BNC/CLI, and BNC/NO was 35%, 61% and 97%, respectively, compared to BNC. BNC/NO showed biocompatibility similar to that of BNC (p = 0.78). BNC/CLI showed the largest inhibition halos, and was superior to the other BNC membranes against S. sanguinis (p < 0.05). The experimental BNC membranes inhibited biofilm formation, with about a 3-fold log CFU reduction compared to BNC (p < 0.05). Conclusions: BNC/NO showed excellent biocompatibility and inhibited multispecies biofilm formation, similarly to BNC/CLI and BNC/CHX.
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da Silva Mairton Gomes,Gheyi Hans Raj,da Silva Lucas Lesqueves,de Souza Thaisa Teixeira,Silva Petterson Costa Conceição,Queiroz Laila de Andrade,dos Santos Thainan Sipriano,Soares Tales Miler 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1
Hydroponics has represented an emerging solution for regions with freshwater scarcity. Besides requiring a lower volume of water for production, this cultivation technique allows the use of brackish waters with high salinity levels. Under normal cultivation conditions (i.e., without any environmental control), plants are exposed to other abiotic stresses, such as tem perature, which can further enhance the salinity eff ect. Given the above, three experiments were conducted using a nutrient f i lm technique (NFT) hydroponic system: from April to May (autumn) with endive ( Cichorium endivia L., cv. Gigante Barbarella), from May to July (autumn/winter) with lettuce ( Lactuca sativa L., cv. Rubinela and cv. Veneranda), and from August to October (winter/spring) with chicory ( Cichorium intybus L., cv. Pão de Açúcar and cv. Radiche Todo Ano), in 2021. The trials aimed to evaluate the isolated and/or combined eff ect of salt and root-zone temperature stresses on yield, water use effi ciency, and visual quality of these vegetables. In all experiments, plants were subjected to stresses for 25 days in a randomized block design with a 2 × 4 factorial arrangement: two levels of electrical conductivity of water –ECw (0.30 and 5.50 dS m –1 for endive and chicory; 0.25 and 6.50 dS m −1 for lettuce) combined with four root-zone temperatures – RZTs (ambient-ARZT, ARZT + 2 °C, ARZT + 4 °C, and ARZT + 6 °C, corresponding to the mean temperatures of 24.72, 26.90, 28.77, and 30.73 °C for endive; 24.20, 26.00, 28.00, and 29.99 °C for lettuce; 27.12, 28.93, 31.01, and 32.86 °C for chicory). The isolated eff ects of RZT stresses or ECw levels on endive traits were observed, with leaf fresh matter losses of approxi mately 17 and 44% at higher ARZT + 6 °C (mean of 30.73 °C) and salinity (ECw 5.50 dS m –1 ), respectively. Shoot fresh matter losses of around 32 and 52% (Veneranda and Rubinela lettuces, respectively) and 65% (Radiche Todo Ano chicory) occurred only due to salt stress. The interaction aff ected the shoot fresh matter of Pão de Açúcar chicory, with approximate losses of 80% at higher ARZT + 6 °C (mean of 32.86 °C) and salt stress (ECw 5.50 dS m –1 ) than ARZT and ARZT + 2 °C and without salt stress (ECw 0.30 dS m –1 ). Despite these reductions, visual quality of plants was not depreciated. Therefore, the present study suggested cultivating leafy vegetables such as lettuce, chicory, and endive using brackish waters, even under high temperatures of nutrient solutions.
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