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Tatjana Stević,Katarina Savikin,Gordana Zdunic,Tatjana Stanojkovic´,Zorica Juranic,Teodora Jankovic,Nebojsˇa Menkovic 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.3
Antioxidant, cytotoxic, and antimicrobial activities of leaves, bark, and cone extracts of Alnus incana (L.) Moench ssp. incana and endemic species A. viridis (Chaix) DC ssp. viridis were evaluated. All extracts were found to be strong 1,1-diphenyl-2-picrylhydrazyl free radical scavengers, exhibiting 50% inhibitory concentration (IC50) values of 3.3–18.9μg/mL, and also showed activity in inhibition of lipid peroxidation with IC50 values ranging from 38.5 to 157.4μg/mL. A. incana and A. viridis extracts exhibited significant cytotoxic effects toward HeLa cells, with IC50 values ranging from 26.02 to 68.5μg/mL. The most active extract of A. incana bark also contained great amounts of total phenolics (316.2mg of gallic acid equivalents/g). In our experiment all extracts were virtually nontoxic on brine shrimps. Extracts were screened for activity against 15 microorganisms, and all extracts investigated showed antimicrobial activity. The most active were dry extracts of cones of A. incana and A. viridis with minimum inhibitory concentration values ranging from 0.117 to 0.129mg/mL.
Cytotoxic and Antimicrobial Activities of Cantharellus cibarius Fr. (Cantarellaceae)
Marina Kolundzic,Tatjana Stanojkovic,Jelena Radovic,Ana Tacic,Margarita Dodevska,Marina Milenkovic,Francesca Sisto,Carla Masia,Giampietro Farronato,Vesna Nikolic,Tatjana Kundakovic 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.8
Antibacterial and cytotoxic activities of cyclohexane, dichloromethane, methanol, and aqueous extracts of Cantharellus cibarius were tested. Broth microdilution assay was performed against 10 bacterial strains (Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Bacillus subtilis, Enterococcus feacalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella abony), with emphasis on Helicobacter pylori. Methanol extract was the most active against H. pylori strains with minimal inhibitory concentration values between 4 and 32 μg/mL. All extracts were active against antibiotic resistant H. pylori. Methanol and aqueous extracts had no cytotoxicity against tested cell lines, whereas cyclohexane and dichloromethane extracts were active against HeLa and N87 cells, but also against healthy MRC-5 cells (IC50 39.26 ± 1.24–134.79 ± 0.01 μg/mL). The tested aqueous extracts have shown 68% of angiotensin-converting enzyme inhibitory activity in doses of 1.25 mg/mL. Chemical analysis has shown the presence of linoleic, cis-vaccenic, and oleic acids, sterols, β-glucans, and polyphenolic compounds.