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Das Prapty,Hasnu Sneha,Lahkar Lipika,Mahanta Saurov,Borthakur Sashin Kumar,Tanti Bhaben 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.4
Brucea mollis Wall. ex Kurz is a potent medicinal plant occurring on hill slopes of Karbi Anglong district of Assam. The species is popularly known to have antimalarial activity in traditional medicine by a few ethnic communities of Northeastern regions. In the present study, an attempt was made to study the antimicrobial and antioxidant activity of B. mollis. The antibacterial and antifungal activity of plant extracts was screened by the disc diffusion method of Kirby-Bauer sensitivity test against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Serratia marcescens, Aspergillus fumigatus and Aspergillus niger. Among all the extracts (leaf ethanol, leaf methanol, callus ethanol, and callus methanol), the callus ethanol extracts was found to exhibit better antimicrobial activity against all the bacterial strains at 20 mg/ml concentration. Callus ethanol extracts exhibited maximum inhibition 17.33 ± 0.33 mm against B. subtilis. In case of antifungal activity, leaf methanol extracts exhibited 17.66 ± 0.33 mm against A. fumigatus and leaf ethanol extracts exhibited 18.33 ± 0.33 mm inhibition against A. niger at 20 mg/ml. The minimum inhibitory concentration (MIC) of the both leaves and callus against the bacterial and fungal strains exhibited lowest 0.156 mg/ml MIC against (A) niger from leaf methanol extract; against (B) subtilis, S. aureus and A. fumigatus from callus ethanol extract and against S. marcescens from callus methanol extract. Moreover, DPPH, Hydrogen peroxide (H2O2) radical scavenging activity, reducing power assay, total phenolic, and flavonoid content were investigated. Both the extracts showed antioxidant activity in a dose-dependent manner and ethanolic extract exhibited better antioxidant properties than methanolic extract.
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Bommisetty Reddyyamini,Chakravartty Navajeet,Hariprasad K. R.,Rameshbabu P.,Sudhakar P.,Bodanapu Reddaiah,Naik Jeevula Bhukya,Reddy B. V. Bhaskar,Lekkala Sivrama Prasad,Gupta Saurabh,Tanti Bhaben,Lach 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2
The grain number is one of the highly complex and crucial traits determining the grain yield in rice. To identify grain number QTLs in rice, we employed the QTL-seq approach in the 297 F2 population derived from the cross between BPT5204 and NLR33892. In this investigation, five regions viz., qGN1, qGN3, qGN7, qGN9, and qGN12 on chromosomes 1,3,7,9, and 12, respectively, were identified as QTLs governing grain number per panicle in rice. To verify the QTLs identified in the present study, traditional QTL mapping was carried out using InDel markers and previously reported SSRs in the QTL region. From qGN12 QTL, RM6953 showed a significant association with the number of grains per panicle with a phenotypic variance of 24.58, 22.10, and 17.20% in F2, F2:3, and BC1F2 populations, respectively, at < 0.0001 P value, indicating major QTL. From genome resequencing data, a missense variance was observed in the exonic region of LOC_Os12g39330 gene controlling AP2 domain-containing protein which plays an important role in seed development in rice. Hence, this QTL can be a potential target for map-based cloning and marker-assisted transfer to enhance the grain number in rice.
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