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You, D.J.,Jongruja, N.,Tannous, E.,Angkawidjaja, C.,Koga, Y.,Kanaya, S. Academic Press 2014 Journal of structural biology Vol.187 No.2
RNase H1 from extreme halophilic archaeon Halobacterium sp. NRC-1 (Halo-RNase H1) requires ≥2M NaCl, ≥10mM MnCl<SUB>2</SUB>, or ≥300mM MgCl<SUB>2</SUB> for folding. To understand the structural basis for this salt-dependent folding of Halo-RNase H1, the crystal structure of Halo-RNase H1was determined in the presence of 10mM MnCl<SUB>2</SUB>. The structure of Halo-RNase H1 highly resembles those of metagenome-derived LC11-RNase H1 and Sulfolobus tokodaii RNase H1 (Sto-RNase H1), except that it contains two Mn<SUP>2+</SUP> ions at the active site and has three bi-aspartate sites on its surface. To examine whether negative charge repulsion at these sites are responsible for low-salt denaturation of Halo-RNase H1, a series of the mutant proteins of Halo-RNase H1 at these sites were constructed. The far-UV CD spectra of these mutant proteins measured in the presence of various concentrations of NaCl suggest that these mutant proteins exist in an equilibrium between a partially folded state and a folded state. However, the fraction of the protein in a folded state is nearly 0% for the active site mutant, 40% for the bi-aspartate site mutant, and 70% for the mutant at both sites in the absence of salt. The active site mutant requires relatively low concentration (~0.5M) of salt for folding. These results suggest that suppression of negative charge repulsion at both active and bi-aspartate sites by salt is necessary to yield a folded protein.
Challenges and Tribulations in the Commercialization of New Crops: Aromatic and Medicinal Plants
Simon, James E.,Jefthas, Elton,Longenhaven, Petrus,Smith, Marianna,Renaud, Erica,Juliani, Rodolfo,Wang, Mingfu,Zimba, Noah,Acquaye, Dan,Park, Chung-Heon,Raskin, Ilya,Tannous, Pierre The Korean Society of Medicinal Crop Science 2002 韓國藥用作物學會誌 Vol.10 No.4
Integrated Kidney Exosome Analysis for the Detection of Kidney Transplant Rejection
Park, Jongmin,Lin, Hsing-Ying,Assaker, Jean Pierre,Jeong, Sangmoo,Huang, Chen-Han,Kurdi, Ahmed,Lee, Kyungheon,Fraser, Kyle,Min, Changwook,Eskandari, Siawosh,Routray, Sujit,Tannous, Bakhos,Abdi, Reza,R American Chemical Society 2017 ACS NANO Vol.11 No.11
<P>Kidney transplant patients require life-long surveillance to detect allograft rejection. Repeated biopsy, albeit the clinical gold standard, is an invasive procedure with the risk of complications and comparatively high cost. Conversely, serum creatinine or urinary proteins are noninvasive alternatives but are late markers with low specificity. We report a urine-based platform to detect kidney transplant rejection. Termed iKEA (integrated kidney exosome analysis), the approach detects extracellular vesicles (EVs) released by immune cells into urine; we reasoned that T cells, attacking kidney allografts, would shed EVs, which in turn can be used as a surrogate marker for inflammation. We optimized iKEA to detect T-cell-derived EVs and implemented a portable sensing system. When applied to clinical urine samples, iKEA revealed high level of CD3-positive EVs in kidney rejection patients and achieved high detection accuracy (91.1%). Fast, noninvasive, and cost-effective, iKEA could offer new opportunities in managing transplant recipients, perhaps even in a home setting.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2017/ancac3.2017.11.issue-11/acsnano.7b05083/production/images/medium/nn-2017-050832_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn7b05083'>ACS Electronic Supporting Info</A></P>
Challenges and Tribulations in the Commercialization of New Crops: Aromatic and Medicinal Plants
James E. Simon,Elton Jefthas,Petrus Longenhaven,Marianna Smith,Erica Renaud,Rodolfo Juliani,Mingfu Wang,Noah Zimba,Dan Acquaye,Chung-Heon Park,Ilya Raskin,Pierre Tannous 韓國藥用作物學會 2002 한국약용작물학회지 Vol.10 No.4
Kodack, D. P.,Chung, E.,Yamashita, H.,Incio, J.,Duyverman, A. M. M. J.,Song, Y.,Farrar, C. T.,Huang, Y.,Ager, E.,Kamoun, W.,Goel, S.,Snuderl, M.,Lussiez, A.,Hiddingh, L.,Mahmood, S.,Tannous, B. A.,Eic Proceedings of the National Academy of Sciences 2012 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.109 No.45