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Lee, Eun-Hye,Kamigaito, Yoshiki,Tsujimoto, Takashi,Seki, Shu,Uyama, Hiroshi,Tagawa, Seiichi,Sung, Moon-Hee The Society of Fiber Science and Technology, Japan 2010 纖維學會誌 Vol.66 No.4
<P>Poly(γ-glutamic acid) (PGA) was crosslinked by γ-ray irradiation to produce the transparent PGA hydrogel. The effects of the molecular weight of PGA and the dosage of the γ-ray irradiation on the swelling ability and mechanical strength of the PGA hydrogel were systematically investigated. The preparation of PGA hydrogel / apatite composite was performed by soaking the hydrogel in a simulated body fluid. The hydrogel obtained from the calcium form of PGA became turbid during the soaking to form the PGA composite. The FT-IR and XRD analyses showed the formation of the crystalline apatite in the PGA hydrogel. The surface morphology of the composite was observed by SEM. Proliferation of MC3T3-E1 cells on the resulting composite was examined for application of tissue engineering scaffold.</P>
( Eun Hye Lee ),( Moon Hee Sung ),( Hiroshi Uyama ),( Takashi Tsujimoto ),( Yoshiki Kamigaito ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.10
A poly(γ-glutamic acid) (γPGA)-cholesterol conjugate was synthesized and its properties were then evaluated. The conjugate exhibited an amphiphilic nature derived from the hydrophilic γPGA backbone and the hydrophobic cholesterol side chain. The conjugate spontaneously formed nanoparticles, becoming an aqueous solution when at low concentrations, and at high concentrations the result was the formation of a physical gel. By utilizing the selfaggregating properties of the conjugate in water, an artificial chaperone was developed. A complex of protein, with the nanoparticles of the conjugate, was formed and the protein was released upon the dissociation of the nanoparticles through the addition of β-cyclodextrin. For denatured carbonic anhydrase, the activity was recovered in the artificial chaperone of the nanoparticle conjugate.