http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
SYMPOSIUM 4 : Molecular Biology and Experimental Models of HCV
( Takaji Wakita ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Since the discovery of the hepatitis C virus (HCV) in 1989, it has been recognized as a serious medical and public health problem worldwide. Most recent large advance in the HCV research is the introduction of NS3 protease inhibitors for clinical use. Rapid progress in HCV research field has been largely dependent of the development of HCV replicon and culture system. We isolated full-length HCV cDNA of JFH-1 strain from a fulminant hepatitis patient. The JFH-1 strain replicated efficiently in cultured cell lines without acquiring adaptive mutations and secreted infectious viral particles into culture medium. This infectious HCV system provides for the first time a powerful tool with which to study the viral life cycle, to construct anti-viral strategies and to develop effective vaccines. HCV culture system has been used for the detailed analysis of HCV life cycle. The results obtained from these studies were necessary to develop novel anti-viral compounds and prophylaxis vaccines for HCV infection. Anti-viral screenings were performed using HCV replicon and infectious systems targeting the viral entry, RNA replication, virus particle assembly and egress steps of HCV life cycles. Several classes of anti-viral compounds were identified and further analyzed for their mechanisms of actions. Furthermore, it is important to develop HCV replication systems with genotype except 1 and 2 because genotype 3 and 4 HCV infections have been spread in many developing countries.
Monitoring the Antiviral Effect of Alpha Interferon on Individual Cells
Kim, Chon Saeng,Jung, Jong Ha,Wakita, Takaji,Yoon, Seung Kew,Jang, Sung Key American Society for Microbiology 2007 Journal of virology Vol.81 No.16
<B>ABSTRACT</B><P>An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and <I>Renilla</I> luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-α) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-α were observed among HCV-containing cells.</P>
Park, Chul-Yong,Jun, Hyun-Jeong,Wakita, Takaji,Cheong, Jae Hun,Hwang, Soon B. American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.14
<P>Hepatitis C virus (HCV) infection is often associated with hepatic steatosis and yet the molecular mechanisms of HCV-associated steatosis are poorly understood. Because sterol regulatory element-binding proteins (SREBPs) are the major transcriptional factors in lipogenic gene expression including fatty acid synthase (FAS), we examined the effects of HCV nonstructural proteins on the signaling pathways of SREBP. In this study, we demonstrated that HCV nonstructural 4B (NS4B) protein increased the transcriptional activities of SREBPs. We also showed that HCV NS4B enhanced the protein expression levels of SREBPs and FAS. This was further confirmed in the context of viral RNA replication and HCV infection. The up-regulation of both SREBP and FAS by NS4B protein required phosphatidylinositol 3-kinase activity. We also demonstrated that NS4B protein induced a lipid accumulation in hepatoma cells. In addition, NS4B protein synergistically elevated the transcriptional activity of HCV core-mediated SREBP-1. These results strongly suggest that NS4B may play an important role in HCV-associated liver pathogenesis by modulating the SREBP signaling pathway.</P>
X-ray Crystallographic Studies on Binding Specificity of Norovirus to Lewis Antigens
Tomomi Kubota,Akiko Kumagai,Hiromi Ito,Sanae Furukawa,Yuichi Someya,Koji Ishii,Takaji Wakita,Naokazu Takeda,Haruko Shirato,Hisashi Narimatsu 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Norovirus (NoV) is a major causative agent of nonbacterial acute, epidemic gastroenteritis, and often widespread in winter. Although most NoV infections are cured without serious symptoms, it could cause severe symptoms in elderlies and young children. The initial infection event is adhesion of NoV to glycans expressed in the epidermal cells of the small intestine. We have identified several NoV strains that show affinity to Le