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RISS 인기검색어
- 검색키워드
- 저자 : Syed Ali Mohamed Yacoob (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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Jairaman, Chitra,Yacoob, Syed Ali Mohamed,Venkatraman, Anuradha,Nagarajan, Yogananth,Murugesan, Gnanadesigan KOREAN PHARMACOPUNCTURE INSTITUTE 2019 Journal of pharmacopuncture Vol.22 No.1
Objective: The aim of the study was to evaluate the hepatoprotective activity of the bark extract (Ethanol: Water) in the ratio of (3:1) of Rhizophora mucronata (BERM) by intoxicating the $HepG_2$ cell lines with different toxicants viz, $CCL_4$, Ethanol and Paracetamol with different concentrations of the extract were used. The $HepG_2$ cell lines were subjected to MTT Assay for studying the cytotoxicity. Methods: $HepG_2$ cells were plated using 96 well plate in 10% bovine serum, exposed to different toxicants viz, 2% $CCl_4$, 60% Ethanol and 14 mM Paracetamol respectively. The various test concentrations (18.85, 37.5, 75, 150 and $300{\mu}g/ml$) of bark extract of Rhizophora mucronata was added and incubated for 24 hours. Medium was removed after incubation period and 0.5 mg/ml MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added and again incubated for 4 hours at 37oC. Then MTT was removed the crystals was dissolved in DMSO and absorbance was measured at 570 nm. Results: The result showed that dose dependent increase in percentage of viability at the doses of 18.85, 37.5, 75, 150, $300{\mu}g/ml$. Te results for the $CCl_4$ intoxicated, at $300{\mu}g/ml$ of the concentration of the extract, the % of viable cells was found out to be 99.6%, for Ethanol intoxicated, 97.67%, and Paracetamol induced, 75.37%, IC50 was $21.53{\mu}g/ml$, $12.61{\mu}g/ml$ and $21.42{\mu}g/ml$ respectively. Conclusion: Thus, we conclude that, the extract possesses defensive effect against different toxicants and can be used as an alternate drug for hepatotoxicity.
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Chitra Jairaman,Syed Ali Mohamed Yacoob,Anuradha Venkatraman,Yogananth Nagarajan,Gnanadesigan Murugesan 대한약침학회 2019 Journal of pharmacopuncture Vol.22 No.1
Objective: The aim of the study was to evaluate the hepatoprotective activity of the bark extract (Ethanol:Water) in the ratio of (3:1) of Rhizophora mucronata (BERM) by intoxicating the HepG 2 cell lines with different toxicants viz, CCL 4 , Ethanol and Paracetamol with different concentrations of the extract were used. The HepG 2 cell lines were subjected to MTT Assay for studying the cytotoxicity. Methods: HepG 2 cells were plated using 96 well plate in 10% bovine serum, exposed to different toxicants viz, 2% CCl 4 , 60% Ethanol and 14 mM Paracetamol respectively. The various test concentrations (18.85, 37.5, 75, 150 and 300 μg/ml) of bark extract of Rhizophora mucronata was added and incubated for 24 hours. Medium was removed after incubation period and 0.5 mg/ml MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added and again incubated for 4 hours at 37oC. Then MTT was removed the crystals was dissolved in DMSO and absorbance was measured at 570 nm. Results: The result showed that dose dependent increase in percentage of viability at the doses of 18.85, 37.5, 75, 150, 300 μg/ml. The results for the CCl4 intoxicated, at 300 μg/ml of the concentration of the extract, the % of viable cells was found out to be 99.6%, for Ethanol intoxicated, 97.67%, and Paracetamol induced, 75.37%, IC50 was 21.53 μg/ml, 12.61 μg/ml and 21.42 μg/ml respectively. Conclusion: Thus, we conclude that, the extract possesses defensive effect against different toxicants and can be used as an alternate drug for hepatotoxicity.
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