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Su Ryun Choi,Sang Heon Oh,Parameswari Paul,Sushil Satish Chhapekar,Jana Jeevan Rameneni,Vignesh Dhandapani,Song Yeon Han,Chang Yeol Lee,Gyung Ja Choi,Yong Pyo Lim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Clubroot (CR) caused by Plasmodiophora brassicae is a severe disease that decreases crop quality and productivity in cruciferous crops. Clubroot resistance linked quantitative trait loci and candidate genes have been identified during last two decades. However, disease lead crop damage continues to occur owing to differences in host variety and constant pathogen variation. Therefore, continued development of markers is required, and the underlying regulatory mechanisms such as the interrelationships between genes and the way in which genes are regulated need to be investigated. MicroRNAs (miRNAs) are attracting attention as regulators of gene expression, including during biotic stress responses. We try to understand how miRNAs regulate clubroot resistance-related genes in P. brassicae-infected Brassica rapa. Two Brassica miRNAs, Bra-miR1885a and Bra-miR1885b, were revealed to target TIR-NBS genes. In non-infected plants, both miRNAs were expressed at low levels to maintain the balance between plant development and basal immunity. However, their expression levels increased in P. brassicae-infected plants. Both miRNAs down regulated the expression of the TIR-NBS genes Bra019412 and Bra019410, which are located at a clubroot resistance-related quantitative trait locus. A qRT-PCR analysis revealed Bra019412 expression was negatively regulated by miR1885. A 5-prime rapid amplification of cDNA ends analysis confirmed the cleavage of Bra019412 by Bra-miR1885b. Thus, miR1885s potentially regulate CR resistance associated TIR-NBS gene expression during P. brassicae infections of B. rapa. Additionally, we have identified the candidate genes against ‘Banglim’ pathotype by fine-mapping with two inbred line 09CR500 and 09CR501. And we developed the novel gene-based markers based on ORF1, ORF2, ORF3 encoding TNL. And using diverse public available information, we have tested the marker availability with core collection population and improved. Those marker information will be useful in Brassica breeding programs such as marker-assisted selection and gene pyramiding to identify and develop resistant line and cultivars.