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Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10
Thi Thu Huong Luong,Supattra Poeaim,Narumon Tangthirasunun 한국균학회 2023 Mycobiology Vol.51 No.4
Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles,and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi wereisolated, and their xylanase activities were tested by the qualitative method. The resultshowed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI)of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitativetest, isolate SP10 performed the highest xylanase activity with 0.535U/mL. Through molecularmethods using the b-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanaseisoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGEindicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, itsxylanase worked optimally at pH 6 and 55 C while reaching 61% activity at 65 C. Theseresults proposed P. menonorum SP10 as a good candidate for industrial uses, especially inpoultry feed and pulp industries, to improve yield and economic efficiency under slightlyacidic and high-temperature conditions.
( Phung Manh Hung ),( Pongnak Wattanachai ),( Soytong Kasem ),( Supattra Poeaim ) 한국균학회 2015 Mycobiology Vol.43 No.3
Thailand is one of the largest citrus producers in Southeast Asia. Pathogenic infection by Phytophthora, however, has become one of major impediments to production. This study identified a pathogenic oomycete isolated from rotted roots of pomelo (Citrus maxima) in Thailand as Phytophthora nicotianae by the internal transcribed spacer ribosomal DNA sequence analysis. Then, we examined the in vitro and in vivo effects of Chaetomium globosum, Chaetomium lucknowense, Chaetomium cupreum and their crude extracts as biological control agents in controlling this P. nicotianae strain. Represent as antagonists in biculture test, the tested Chaetomium species inhibited mycelial growth by 50~56% and parasitized the hyphae, resulting in degradation of P. nicotianae mycelia after 30 days. The crude extracts of these Chaetomium species exhibited antifungal activities against mycelial growth of P. nicotianae, with effective doses of 2.6~101.4 μg/mL. Under greenhouse conditions, application of spores and methanol extracts of these Chaetomium species to pomelo seedlings inoculated with P. nicotianae reduced root rot by 66~71% and increased plant weight by 72~85% compared to that in the control. The method of application of antagonistic spores to control the disease was simple and economical, and it may thus be applicable for large-scale, highly effective biological control of this pathogen.