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Expression of Heterologous Bacterial CO₂-fixation Pathway in Yeast
Sujeong PARK,Deokyeol JEONG,Suhyeung KIM,Bonbin GU,Soo Rin KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Unpredictable climate change caused by global warming is threatening humanity and the ecosystem. To solve this problem, more than 110 countries and international organizations have agreed to reduce carbon dioxide (CO₂) emissions. Recently various industrial processes such as bioethanol and chemical production rely on yeast fermentation. In the yeast fermentation process, CO₂ emission into atmosphere is unavoidable. Therefore, expressing CO₂ fixation pathway in yeast would decrease the CO₂ emission during the process. The purpose of this study is to develop a S. cerevisiae strain that can utilize CO₂ generated during the fermentation. First, we constructed xylose oxidoreductase pathway to produce the precursor of CO₂ fixation pathway. Then, heterologous genes related to CO₂ fixation pathway are introduced by the CRISPR/Cas9 genome editing technology. We confirmed the soluble expression of ribulose-1,5-bisphosphate carboxylase/oxygenase. Moreover, we observed increased ethanol yield through the fermentation. These results suggest that expressing CO₂ fixation pathway in S. cerevisiae could be a strategy to alleviate the global warming.
Development of a meso-galactarate Production Platform in Engineered Yeast
Deokyeol JEONG,Heeyoung PARK,Sujeong PARK,Suhyeung KIM,Byeong-Kwan JANG,Je Min LEE,Soo Rin KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
meso-galactarate, a platform chemical of bioplastic, is converted by some pathogenic bacterial uronate dehydrogenase (udh) from D-galacturonic acid, which is abundantly present in pectin-rich biomass. Although microbial meso-galactarate production has been mostly studied based on filamentous fungi, an optimal platform has not been developed for yeast Saccharomyces cerevisiae, despite its large capacities such as high acid tolerance and rapid growth. The purpose of present study is to develop an S. cerevisiae strain that can efficiently convert meso-galactarate. Three different udh genes and overexpression of one of them were completely introduced by Cas9-genome editing in S. cerevisiae, allowed the conversion of meso-galactarate completely without specific transporter of D-galacturonic acid. Moreover, the additional expression of the dicarboxylic acid transporter gene in the meso-galactarate producing strain showed higher meso-galactarate production efficiency. The final engineered strain showed a high titer of meso-galactarate from citrus peel waste hydrolysate. Through this study, we can suggest that the possibility as a platform host to utilize pectin-rich biomass.