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        Polyphyletic photosynthetic reaction centre genes in oligotrophic marine <i>Gammaproteobacteria</i>

        Cho, Jang-Cheon,Stapels, Martha D.,Morris, Robert M.,Vergin, Kevin L.,Schwalbach, Michael S.,Givan, Scott A.,Barofsky, Douglas F.,Giovannoni, Stephen J. BLACKWELL SCIENCE 2007 ENVIRONMENTAL MICROBIOLOGY Vol.9 No.6

        <P>Summary</P><P>Ecological studies indicate that aerobic anoxygenic phototrophic bacteria (AAP) that use bacteriochlorophyll to support phototrophic electron transport are widely distributed in the oceans. All cultivated marine AAP are alpha-3 and alpha-4 <I>Proteobacteria</I>, but metagenomic evidence indicates that uncultured AAP <I>Gammaproteobacteria</I> are important members of ocean surface microbial communities. Here we report the description of obligately oligotrophic, marine <I>Gammaproteobacteria</I> that have genes for aerobic anoxygenic photosynthesis. Three strains belonging to the OM60 clade were isolated in autoclaved seawater media. Polymerase chain reaction assays for the <I>puf</I>M gene show that these strains contain photosynthetic reaction centre genes. DNA sequencing and phylogenetic analysis indicate that the <I>puf</I>M genes are polyphyletic, suggesting multiple instances of lateral gene transfer. Peptide sequences from six photosynthesis genes (<I>puf</I>L, <I>puf</I>M, <I>puf</I>C, <I>puf</I>B, <I>puf</I>A and <I>puh</I>A) were detected by proteomic analyses of strain HTCC2080 cells grown aerobically in seawater. They closely match predicted peptides from an environmental seawater bacterial artificial chromosome clone of gammaproteobacterial origin, thus identifying the OM60 clade as a significant source of gammaproteobacterial AAP genes in marine systems. The cell yield and rate of growth of HTCC2080 in autoclaved, aerobic seawater increased in the light. These findings identify the OM60 clade as a source of <I>Gammaproteobacteria</I> AAP genes in coastal oceans, and demonstrate that aerobic, anoxygenic photosynthetic metabolism can enhance the productivity of marine oligotrophic bacteria that also grow heterotrophically in darkness.</P>

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        Overlapping regionalism around the world: Introducing the overlapping regionalism dataset

        Panke Diana,Stapel Sören 한국외국어대학교 국제지역연구센터 2023 International Area Studies Review Vol.26 No.4

        Regional cooperation often leads to the establishment of regional international organizations (RIOs). Due to the increasing number of RIOs, their growing membership size and their broadening policy scope, RIOs frequently overlap with each other with regard to membership and mandate. Overlaps may lead to conflicts amongst affected organizations and potentially limit the prospects for regional prosperity and peace. Although overlapping regionalism has become ubiquitous in many regions, we do not systematically know the extent of the phenomenon. Therefore, the paper presents an original dataset about overlapping regionalism and introduces a typology of overlaps between two RIOs. The OVREG dataset includes both raw data of shared member states and policy competencies and information about distinct types of overlap between affected RIOs for 73 RIOs, 193 states and 344 policy competencies in the period between 1945 and 2020. A descriptive analysis of trajectories and patterns shows that overlapping regionalism in general and the different overlap types have spread over time and across world regions. Nevertheless, regional particularities persist. Thus, the OVREG dataset allows for examining the causes of overlaps, the potential negative consequences of overlapping regionalism and the variety of coping mechanisms at the disposal of affected RIOs.

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