Preliminary Linkage Mapping of Tea Plants with RAPD and SSR Markers

Yali Chang,Eun Ui Oh,Min Seuk Lee,In Kwan Song,Joo Yoo,Kwan Jeong Song 한국차학회 2015 한국차학회지 Vol.- No.S

Based on double pseudo-testcross theory, a population of 76 F1 clones derived from crossing between female parent ‘Kemsull’ and male parent ‘Houshun’ was used to construct the linkage map using RAPD and SSR markers. Totally 660 random primers with 10 nucleotides were screened and 44 was selected, 60 pairs of SSR primers were screened and 14 pairs were chosen. All 142 RAPD and 14 SSR markers amplified by selected primers weresorted out into two data sets obtained from each parent, then grouped by JoinMap 4.0 version. We constructed the ‘Kemsull’ map containing 77 RAPD markers and 5 pairs of SSR markers into 14 linkage groups (495.1 cM), and the ‘Houshun’map containing 81 RAPD markers and 7 SSR markers into 13 lingkage groups (431.5 cM) (n = 15 in tea). The map of ‘Houshun’ was consisted of the longest linkage group 4 (76.1 cM) with only 2 intervals and the shortest linkage group 2 (10.2 cM, excepted three zero cM linkage groups), with a mean length of 33.2 cM. The map of ‘Kemsull’ was consisted of the longest linkage group (76.1 cM) with 5 intervals and the shortest linkage group 6 (20.8 cM, excepted four zero cM linkage groups). Since maps were not saturated with RAPD and SSR markers, these preliminary linkage maps should be improved by adding other molecular markers.

A Genetic Linkage Map based on AFLP markers in China type Tea Plant

Yali Chang,Eun‐Ui Oh,Min‐Seuk Lee,Kwan‐Jeong Song 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Based on double pseudo-testcross theory, a population of 76 F1 clones, which were derived from a cross of China type tea plants (Camellia sinensis var. sinensis) with a Korean tea cultivar, ‘Kemsull’ for female parent and a Japanese tea cultivar, ‘Houshun’ for male parent, was used to construct a genetic linkage map with AFLP markers. Totally, 2,360 markers were detected by 26 pairs of primers and 90.8 markers for each pair on average. Among these, 481 markers (20.3%) were polymorphic, 392 markers (81.5%) of which showed Mendelian segregation ratio (p=0.01). Of these Mendelian segregated markers, 139 (35.5%) were segregated in 3:1 ratio and 253 (65.5%) were segregated in 1:1 ratio. The construction of AFLP molecular marker based linkage map were carried out by Joinmap 4.0 version. The linkage map of ‘Kemsull’ contained 227 markers which distributed into 18 linkage groups. The linkage map of ‘Kemsull’ covered 1,382.2 cM with the average distance between two markers of 6.0 cM. The linkage map of ‘Houshun’ contained 154 markers which were distributed into 17 linkage groups and were spanned with the total map length of 1,540.9 cM and the average distance between two markers of 10 cM. However, these AFLP markers were not distributed evenly and further even saturation is additionally required.

Current Status and Prospect of Molecular Breeding in Tea Plant (Camellia sinensis)

(Yali Chang),(H. M. Prathibhani Chamidha Kumarihami),(Ho Bang Kim),(Kwan Jeong Song) 한국차학회 2017 한국차학회지 Vol.23 No.1

차는 건강 증진 효과는 물론 다양한 질병 예방 효과에 기인하여 세계적으로 가장 일반적으로 소비되는 비알코올 음료이며 많은 국가에서 중요한 소득원의 하나로서 과학 자와 생산자뿐만 아니라 소비자에게도 큰 관심의 대상이 되어 왔다. 그러므로 특정 이차대사산물의 고함유 또는 생물적 및 비생물적 스트레스의 저항성 증진과 같이 국제 차 시장에서 다양한 부가 가치를 줄 수 있는 새로운 차 품종의 육종이 시급히 요구되어 왔다. 전통 육종은 새로운 품종의 성공적인 개발에 매우 중요한 역할을 수행해 오고 있으나 육종 기간이 길고 비용이 많이 든다. 반면, DNA 분자표지의 개발은 육종 기간을 단축하고 차나무의 유전적 개량을 위한 육종 과정의 빠른 진행을 가능하게 해준다. 본 논문에서는 차나무의 유전적 다양성 및 유전분석, 핵심집단 및 유전자원 관리, 유전자 지도 및 분자표지 이용 선발, 그리고 유전체 및 유전자 발현 분석으로 구성하여 차나무 분자 육종과 유전적 개량의 최신 성과와 전망에 대해 전반적으로 고찰하고자 한다. Tea, as one of mostly popular consumed non-alcoholic beverages and an important revenue resource for a number of countries worldwide, has attracted a great deal of attention not only from scientists and growers, but also from consumers, because of its health-promoting function, as well as its protective effects against certain kinds of diseases. Therefore, there has been an enormous demand for breeding new tea cultivars with multiple value orientations in the international tea market, such as a high content of specific secondary metabolites or strong resistance to biotic and abiotic stresses. The conventional breeding process played an important role in the successful selection of new tea cultivars, but is time-consuming and costly. On the other hand, the discovery of DNA molecular markers has provided the opportunity to shorten the breeding time and accelerate the breeding process for improving the genetics of the tea plant. This paper is intended to provide comprehensive coverage of the recent achievements and perspectives in the field of tea plant molecular breeding and genetic improvement, integrated with genetic diversity and genotyping, core collection and genetic resources management, genetic linkage mapping and marker-assisted selection (MAS), as well as genomics and gene expression profiling.

Construction of a genetic linkage map based on RAPD, AFLP, and SSR markers for tea plant (Camellia sinensis)

Chang, Yali,Oh, Eun Ui,Lee, Min Seuk,Kim, Ho Bang,Moon, Doo-Gyung,Song, Kwan Jeong Springer-Verlag 2017 Euphytica Vol.213 No.8

Antihypertensive Effect of Few-flower Wild Rice (Zizania latifolia Turcz.) in Spontaneously Hypertensive Rats

Yun Deng,Yali Luo,Bingjun Qian,Zhenmin Liu,Yuanrong Zheng,Xiaoyong Song,Shaojuan Lai,Yanyun Zhao 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.2

The antihypertensive activity of few-flower wildrice was studied in spontaneously hypertensive rats (SHRs)with evaluation of blood pressure lowering effects andtranscriptional levels of the sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) gene that is regulated by AngiotensinII (Ang II). SHRs were randomly divided into 5 groupswith 6 rats each. The systolic blood pressure (SBP) reachedthe lowest point 3 h after administration of a single dose ofpaste made from few-flower wild rice stem powder. TheSBP of SHR in the relatively high amount of RSP (HRSP)administrated group was reduced by approximately 30mmHg, compared to the negative control group, and wasnot significantly different from the positive control IPPcontrol group at a dose of 1.5 mg/kg body weight (p>0.05). RSP administrated SHRs showed a significantly higherSERCA2a transcription level than negative control SHRs(p<0.05). RSP administration had no negative effects onglycometabolism of SHR.

CCAJS: A Novel Connect Coverage Algorithm Based on Joint Sensing Model for Wireless Sensor Networks

( Zeyu Sun ),( Yali Yun ),( Houbing Song ),( Huihui Wang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.10

This paper discusses how to effectively guarantee the coverage and connectivity quality of wireless sensor networks when joint perception model is used for the nodes whose communication ranges are multi-level adjustable in the absence of position information. A Connect Coverage Algorithm Based on Joint Sensing model (CCAJS) is proposed, with which least working nodes are chosen based on probability model ensuring the coverage quality of the network. The algorithm can balance the position distribution of selected working nodes as far as possible, as well as reduce the overall energy consumption of the whole network. The simulation results show that, less working nodes are needed to ensure the coverage quality of networks using joint perception model than using the binary perception model. CCAJS can not only satisfy expected coverage quality and connectivity, but also decrease the energy consumption, thereby prolonging the network lifetime.

Zinc Oxide-Enhanced Piezoelectret Polypropylene Microfiber for Mechanical Energy Harvesting

Zhu, Jianxiong,Zhu, Yali,Song, Weixing,Wang, Hui,Gao, Min,Cho, Minkyu,Park, Inkyu American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.23

<P>This paper reports zinc oxide (ZnO)-coated piezoelectret polypropylene (PP) microfibers with a structure of two opposite arc-shaped braces for enhanced mechanical energy harvesting. The ZnO film was coated onto PP microfibers via magnetron sputtering to form a ZnO/PP compound structure. Triboelectric Nanogenerator (TENG) based on ZnO/PP microfiber compound film was carefully designed with two opposite arc-shaped braces. The results of this study demonstrated that the mechanical energy collection efficiency of TENG based on piezoelectret PP microfiber was greatly enhanced by the coated ZnO and high-voltage corona charging method. We found that, with the step-increased distance of traveling for the movable carbon black electrode, an electrical power with an approximately quadratic function of distance was generated by this mechanical-electrical energy conversion, because more PP microfibers were connected to the electrode. Further, with a full contact condition, the peak of the generated voltage, current, and charges based on the ZnO/PP microfibers by this mechanical-electrical energy conversion with 1 m/s<SUP>2</SUP> reached 120 V, 3 μA, and 49 nC, respectively. Moreover, a finger-tapping test was used to demonstrate that the ZnO/PP microfiber TENG is capable of lighting eight light-emitting diodes.</P> [FIG OMISSION]</BR>

Exploring effects of different male parent crossings on sheep muscles and related regulatory genes using mRNA-Seq

Shi Jinping,Zhang Quanwei,Song Yali,Lei Zhaomin,Fu Lingjuan,Cheng Shuru 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.8

Objective: With improvements in living standards and increase in global population, the demand for meat products has been increasing; improved meat production from livestock could effectively meet this demand. In this study, we examined the differences in the muscle traits of different male crossbred sheep and attempted to identify key genes that regulate these traits. Methods: Dubo sheep×small-tailed Han sheep (DP×STH) and Suffolk×small-tailed Han sheep (SFK×STH) were selected to determine meat quality and production performance by Masson staining. Transcriptome sequencing and bioinformatic analysis were performed to identify differentially expressed genes (DEGs) related to meat quality. The presence of DEGs was confirmed by real-time polymerase chain reaction. Results: The production performance of SFK×STH sheep was better than that of DP×STH sheep, but the meat quality of DP×STH sheep was better than that of SFK×STH sheep. The muscle fiber diameter of DP×STH sheep was smaller than that of SFK×STH sheep. Twentytwo DEGs were identified. Among them, four gene ontology terms were related to muscle traits, and three DEGs were related to muscle or muscle fibers. There were no significant differences in the number of single nucleotide mutations and mutation sites in the different male parent cross combinations. Conclusion: This study provides genetic resources for future sheep muscle development and cross-breeding research. Objective: With improvements in living standards and increase in global population, the demand for meat products has been increasing; improved meat production from livestock could effectively meet this demand. In this study, we examined the differences in the muscle traits of different male crossbred sheep and attempted to identify key genes that regulate these traits.Methods: Dubo sheep×small-tailed Han sheep (DP×STH) and Suffolk×small-tailed Han sheep (SFK×STH) were selected to determine meat quality and production performance by Masson staining. Transcriptome sequencing and bioinformatic analysis were performed to identify differentially expressed genes (DEGs) related to meat quality. The presence of DEGs was confirmed by real-time polymerase chain reaction.Results: The production performance of SFK×STH sheep was better than that of DP×STH sheep, but the meat quality of DP×STH sheep was better than that of SFK×STH sheep. The muscle fiber diameter of DP×STH sheep was smaller than that of SFK×STH sheep. Twenty-two DEGs were identified. Among them, four gene ontology terms were related to muscle traits, and three DEGs were related to muscle or muscle fibers. There were no significant differences in the number of single nucleotide mutations and mutation sites in the different male parent cross combinations.Conclusion: This study provides genetic resources for future sheep muscle development and cross-breeding research.

Enhancing Electrocatalytic Production of H2O2 by Modulating Coordination Environment of Cobalt Center

Guoling Wu,Zhongjie Yang,Tianlin Zhang,Yali Sun,Chang Long,Yaru Song,Shengbin Lei,Zhiyong Tang 대한화학회 2021 Bulletin of the Korean Chemical Society Vol.42 No.8

As an environmentally friendly oxidant, H2O2 is widely utilized in various fields; however, its production methods remain limited to the chemical anthraquinone process. Alternatively, electrocatalytic oxygen reduction possesses numerous notable advantages (e.g., cost-effectiveness, small-scale, and distributed nature). As electrocatalytic oxygen reduction has been widely investigated in the fields of fuel cells and metal-air batteries, the mechanism of the 2e?-ORR pathway for producing H2O2 is not sufficiently clear. Herein, we explore the effect of the cobalt (Co) coordination environment on the electrochemical production of H2O2. The detailed investigation on N-, P-, and S-coordinated Co catalysts (Co1N1N3, Co1P1N3, and Co1S1N3) demonstrates that changing the coordination environment evidently affects the H2O2 selectivity, and the S-coordinated Co exhibits the best catalytic performance. This finding would lead to the design and selection of catalysts at atomic level for producing H2O2 via electrocatalytic oxygen reduction.

Insight on the microbial activity and microbiome in partial nitrification systems: CuO nanoparticles impact under different pH levels

Xiaojing Zhang,Nan Zhang,Yue Zhou,Robert Delatolla,Yali Song,Yongpeng Ma,Hongzhong Zhang 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.6

In this study, the effect of CuO nanoparticles (NPs) on partial nitrification (PN) process was investigated at various pH values. The short- and long-term experiments were carried out in six identical reactors, with or without CuO NPs, at pH values of 6.5, 8.0 and 10.0. The ammonia oxidation, reaction rates, copper content, and the microbial community were investigated. The results of this work suggested that CuO NPs inhibited the ammonia oxidation by aerobic ammonia-oxidizing bacteria (AOB), under both the acid and alkali conditions. AOB could resist the acid condition but was significantly suppressed when CuO NPs was fed, and the low pH did not aggravate the inhibition level. Almost all the bacteria lost bioactivity under the pH as 10.0, while anaerobic ammonia-oxidizing bacteria survived. The acid condition increased the Nitrosomonas relative abundance while the alkali condition lowered it. More than 60% of the supplied CuO NPs was discharged via effluent.