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Solution and analysis of VL combined seal lubrication model under the effect of wear
Yi Zhang,Siyang Xiong,Sipeng Zhong,Zijie Xiong,Qiang Yang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.11
Through the finite element calculation of VL combined seal models under different wear conditions, contact pressure distributions of models are obtained. Considering the coupling effect between seal deformation and lubricating oil film, a mathematical model of elastohydrodynamic lubrication for VL combined seal is established. Based on the theory of small deformation, the elastic deformation of VL composite seal under high pressure is obtained by the deformation influence coefficient matrix method. Considering the influence of sealing surface wear and surface roughness, the oil film thickness distribution and oil film pressure distribution of VL combined seal are solved by the finite difference method. The analysis results show the wear of VL combined seal, the decrease of viscosity, the increase of roughness and rotational speed can raise the thickness and pressure of lubricating oil film. The correctness of numerical simulation is verified by experimentally measuring the friction torque and leakage rate of seal.
Qiu Mohan,Yu Chunlin,Zhu Shiliang,Liu Siyang,Peng Han,Xiong Xia,Chen Jialei,Jiang Xiaosong,Du Huarui,Li Qingyun,Zhang Zengrong,Yang Chaowu 한국유전학회 2022 Genes & Genomics Vol.44 No.11
Background: Long non-coding RNAs (lncRNAs) play an essential role in biological processes. However, the expression patterns of lncRNAs that regulate the non-Mendelian inheritance feather phenotypes remain unknown. Objective: This study aimed to compare the expression profiles of lncRNAs in the follicles of the late-feathering cocks (LC) and late-feathering hens (LH) that followed genetic rules and the early-feathering hen (EH) and early-feathering cock (EC) that did not conform to the genetic laws. Methods: We performed RNA sequencing and investigated the differentially expressed lncRNAs (DElncRNAs) between the early- and late-feathering chickens, which function by cis-acting or participate in the competing endogenous RNA (ceRNA) network. Results: A total of 53 upregulated and 43 downregulated lncRNAs were identified in EC vs. LC, and 58 upregulated and 109 downregulated lncRNAs were identified in EH vs. LH. The target mRNAs regulated by lncRNAs in cis were enriched in the pentose phosphate pathway, TGF-β signaling pathway and Jak-STAT signaling pathway in EC vs. LC and were associated with the TGF-β signaling pathway, Wnt signaling pathway, p53 signaling pathway and Jak-STAT signaling pathway in EH vs. LH. In addition, the lncRNA-mediated ceRNA regulatory pathways of hair follicle formation were mainly enriched in the TGF-β signaling pathway, Wnt signaling pathway, melanogenesis, and calcium signaling pathways. The levels of ENSGALG00000047626 were significantly higher in the late-feathering chickens than in the early-feathering chickens, which regulated the expression of SSTR2 by gga-miR-1649-5p. Conclusion: This study provides a novel molecular mechanism of lncRNA's response to the feather rate that does not conform to the genetic laws in chickens.