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Jung, K.C.,Simond, D.M.,Moran, C.,Hawthorne, W.J.,Jeon, J.T.,Jin, D.I.,Lee, J.H. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.11
The xenotransplantation of pig organs and cells can be related with a risk of transmission of infectious diseases to human. Previous findings indicate that the regulatory region of PERV for retroviral transcription, replication and integration into the cellular DNA is located on the 5' Long Terminal Repeat (LTR). The objective of this study is the investigation of methylation and deletion status of the PERV 5' LTR region which can be used for regulating PERV expression. We compared the sequences of genomic DNA and bisulfite-treated genomic DNA from PK-15 cells expressing PERV to observe the methylation status of the 5' LTR. Our results showed that the CpG sites of U3 were methylated and methylation was inconsistent in the R and U5 regions. Also, variable numbers of 18 bp repeats and 21 bp repeats were detected on 5' LTR by sequencing analysis. The consistent U3 methylation might be indicative of host suppression of expression of the retroviruses.
Nicolet, Christophe,Alligne, Sebastien,Kawkabani, Basile,Simond, Jean-Jacques,Avellan, Francois Korean Society for Fluid machinery 2009 International journal of fluid machinery and syste Vol.2 No.4
This paper presents a numerical simulation study of the transient behavior of a $2{\times}340MW$ pump-turbine power plant, where the results show an unstable behavior at runaway. First, the modeling of hydraulic components based on equivalent schemes is presented. Then, the 2 pump-turbine test case is presented. The transient behavior of the power plant is simulated for a case of emergency shutdown with servomotor failure on Unit 1. Unstable operation at runaway with a period of 15 seconds is properly simulated using a 1-dimensional approach. The simulation results points out a switch after 200 seconds of the unstable behavior between a period of oscillations initially of 15 seconds to a period of oscillation of 2.16 seconds corresponding to the hydraulic circuit first natural period. The pressure fluctuations related to both the rigid and elastic water column mode are presented for oscillation mode characterization. This phenomenon is described as a switch between a rigid and an elastic water column oscillation mode. The influence of the rotating inertia on the switch phenomenon is investigated through a parametric study.
Alligne, S.,Nicolet, C.,Allenbach, P.,Kawkabani, B.,Simond, J.J.,Avellan, F. Korean Society for Fluid machinery 2009 International journal of fluid machinery and syste Vol.2 No.4
Hydroelectric power plants are known for their ability to cover variations of the consumption in electrical power networks. In order to follow this changing demand, hydraulic machines are subject to off-design operation. In that case, the swirling flow leaving the runner of a Francis turbine may act under given conditions as an excitation source for the whole hydraulic system. In high load operating conditions, vortex rope behaves as an internal energy source which leads to the self excitation of the system. The aim of this paper is to identify the influence of the full load excitation source location with respect to the eigenmodes shapes on the system stability. For this, a new eigenanalysis tool, based on eigenvalues and eigenvectors computation of the nonlinear set of differential equations in SIMSEN, has been developed. First the modal analysis method and linearization of the set of the nonlinear differential equations are fully described. Then, nonlinear hydro-acoustic models of hydraulic components based on electrical equivalent schemes are presented and linearized. Finally, a hydro-acoustic SIMSEN model of a simple hydraulic power plant, is used to apply the modal analysis and to show the influence of the turbine location on system stability. Through this case study, it brings out that modeling of the pipe viscoelastic damping is decisive to find out stability limits and unstable eigenfrequencies.