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Alvarado-Ruiz, Liliana,Martinez-Silva, Maria Guadalupe,Torres-Reyes, Luis Alberto,Pina-Sanchez, Patricia,Ortiz-Lazareno, Pablo,Bravo-Cuellar, Alejandro,Aguilar-Lemarroy, Adriana,Jave-Suarez, Luis Feli Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3
HOX transcription factors are evolutionarily conserved in many different species and are involved in important cellular processes such as morphogenesis, differentiation, and proliferation. They have also recently been implicated in carcinogenesis, but their precise role in cancer, especially in cervical cancer (CC), remains unclear. In this work, using microarray assays followed by the quantitative polymerase chain reaction (qPCR), we found that the expression of 25 HOX genes was downregulated in CC derived cell lines compared with non-tumorigenic keratinocytes. In particular, the expression of HOXA9 was observed as down-modulated in CC-derived cell lines. The expression of HOXA9 has not been previously reported in CC, or in normal keratinocytes of the cervix. We found that normal CC from women without cervical lesions express HOXA9; in contrast, CC cell lines and samples of biopsies from women with CC showed significantly diminished HOXA9 expression. Furthermore, we found that methylation at the first exon of HOXA9 could play an important role in modulating the expression of this gene. Exogenous restoration of HOXA9 expression in CC cell lines decreased cell proliferation and migration, and induced an epithelial-like phenotype. Interestingly, the silencing of human papilloma virus (HPV) E6 and E7 oncogenes induced expression of HOXA9. In conclusion, controlling HOXA9 expression appears to be a necessary step during CC development. Further studies are needed to delineate the role of HOXA9 during malignant progression and to afford more insights into the relationship between downmodulation of HOXA9 and viral HPV oncoprotein expression during cercical cancer development.
Establishment of the Latin American Soil Information System Case Colombia - IRAKA
Araujo-Carrillo Gustavo Alfons,Martinez-Maldonado Fabio Ernes,Varon-Ramirez Viviana Marcela,Gomez-Latorre Douglas Andres,Estupinan-Casallas Jhon Mauric,Silva-Arero Elias Alexander,Gonzalez-Jaimes Edua 한국토양비료학회 2019 한국토양비료학회 학술발표회 초록집 Vol.2019 No.8
Mehdi Ahmadi,Farshid Ghanbari,Alberto Alvarez,Susana Silva Martinez 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.8
The performance of UVA-LEDs assisted peroxymonosulfate (PMS)/Fe2+ system was evaluated on carmoisine (E122) decolorization. Complete color removal was obtained under the conditions of pH=3.0, PMS=1.5mM, Fe2+=1mM and 25 min reaction time. UVA-LEDs were preferable compared to conventional UVA lamp in terms of decolorization. The functions of Co2+, Cu2+, Mn2+ and Fe2+ were compared and their results showed that Co2+ and Fe2+ had the highest efficiencies. Moreover, the presence of chloride ion showed a double role in different concentrations in which promotional effect was observed in 100mM Cl−<, while inhibitory effect occurred in 1-10mM Cl−. However, the high concentration of chloride had no influence on mineralization of E122. The scavenging results demonstrated that the UV irradiation increased the contribution of HO•. In addition, in the presence of chloride ion, HOCl along with sulfate and hydroxyl radicals were the major oxidative agents.
Aleman-Gama, Elizabeth,Cornejo-Martell, Alan J.,Kamaraj, Sathish Kumar,Juarez, Katy,Silva-Martinez, Susana,Alvarez-Gallegos, Alberto The Korean Electrochemical Society 2022 Journal of electrochemical science and technology Vol.13 No.2
The high internal resistance (R<sub>int</sub>) that develops across the sediment microbial fuel cells (SMFC) limits their power production (~4/10 mW m<sup>-2</sup>) that can be recovered from an initial oil-contaminated sediment (OCS). In the anolyte, R<sub>int</sub> is related to poor biodegradation activity, quality and quantity of contaminant content in the sediment and anode material. While on the catholyte, R<sub>int</sub> depends on the properties of the catholyte, the oxygen reduction reaction (ORR), and the cathode material. In this work, the main factors limiting the power output of the SMFC have been minimized. The power output of the SMFC was increased (47 times from its initial value, ~4 mW m<sup>-2</sup>) minimizing the SMFC R<sub>int</sub> (28 times from its initial value, 5000 ohms), following the main modifications. Anolyte: the initial OCS was amended with several amounts of gasoline and kerosene. The best anaerobic microbial activity of indigenous populations was better adapted (without more culture media) to 3 g of kerosene. Catholyte: ORR was catalyzed in birnessite/carbon fabric (CF)-cathode at pH 2, 0.8M Na<sub>2</sub>SO<sub>4</sub>. At the class level, the main microbial groups (Gammaproteobacteria, Coriobacteriia, Actinobacteria, Alphaproteobacteria) with electroactive members were found at C-anode and were associated with the high-power densities obtained. Gasoline is more difficult to biodegrade than kerosene. However, in both cases, SMFC biodegradation activity and power output are increased when ORR is performed on birnessite/CF in 0.8 M Na<sub>2</sub>SO<sub>4</sub> at pH 2. The work discussed here can focus on bioremediation (in heavy OCS) or energy production in future work.
Castiblanco Diana Maryuri Correa,Lima Michele Bernardino de,Artoni Silvana Martinez Baraldi,Raimundo Erikson Kadoshe de Morais,Santos Daniel Silva,Carvalho Lizia Cordeiro de,Silva Edney Pereira da 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.1
Objective: Current quail production is configured as an economic activity in scale. Advancements in quail nutrition have been limited to areas such as breeding and, automation of facilities and ambience. The objective of this study was to evaluate the performance responses, liver and oviduct morphometry, and liver histology of Japanese laying quails subjected to different levels of nitrogen-corrected apparent metabolizable energy (MEn). Methods: A completely random design was used that consisted of nine levels of MEn, six replicates, and five hens per cage with a total of 270 quails. The experimental period lasted for 10 weeks. The variables of performance were subjected to analysis of variance and then regression analysis using the broken-line model. The morphometric and histological variables were subjected to multivariate exploratory techniques. Results: The MEn levels influenced the responses to zootechnical performance. The brokenline model estimated the maximum responses for feed intake, egg production, egg weight, and egg mass as 3,040, 2,820, 1,802, and 2,960 kcal of MEn per kg of diet, respectively. Multivariate analysis revealed that the occurrence of hepatic steatosis and increased levels of Kupffer cells were not related to MEn levels. Conclusion: The level of 2,960 kcal/kg of MEn meets performance variable requirements without compromising hepatic physiology.