http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Anatomy of zebrafish cerebellum and screen for mutations affecting its development
Bae, Young-Ki,Kani, Shuichi,Shimizu, Takashi,Tanabe, Koji,Nojima, Hideaki,Kimura, Yukiko,Higashijima, Shin-ichi,Hibi, Masahiko Elsevier 2009 Developmental biology Vol.330 No.2
<P><B>Abstract</B></P><P>The cerebellum is important for the integration of sensory perception and motor control, but its structure has mostly been studied in mammals. Here, we describe the cell types and neural tracts of the adult zebrafish cerebellum using molecular markers and transgenic lines. Cerebellar neurons are categorized to two major groups: GABAergic and glutamatergic neurons. The Purkinje cells, which are GABAergic neurons, express parvalbumin7, carbonic anhydrase 8, and aldolase C like (zebrin II). The glutamatergic neurons are <I>vglut1</I><SUP><I>+</I></SUP> granule cells and <I>vglut2</I><SUP><I>high</I></SUP> cells, which receive Purkinje cell inputs; some <I>vglut2</I><SUP><I>high</I></SUP> cells are eurydendroid cells, which are equivalent to the mammalian deep cerebellar nuclei. We found <I>olig2</I><SUP><I>+</I></SUP> neurons in the adult cerebellum and ascertained that at least some of them are eurydendroid cells. We identified markers for climbing and mossy afferent fibers, efferent fibers, and parallel fibers from granule cells. Furthermore, we found that the cerebellum-like structures in the optic tectum and antero-dorsal hindbrain show similar Parvalbumin7 and Vglut1 expression profiles as the cerebellum. The differentiation of GABAergic and glutamatergic neurons begins 3?days post-fertilization (dpf), and layers are first detectable 5?dpf. Using anti-Parvalbumin7 and Vglut1 antibodies to label Purkinje cells and granule cell axons, respectively, we screened for mutations affecting cerebellar neuronal development and the formation of neural tracts. Our data provide a platform for future studies of zebrafish cerebellar development.</P>