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He-Xun Huang,Ting Yu,Jun-Xing Li,Shu-Ping Qu,왕만만,Ting-Quan Wu,Yu-Juan Zhong 한국식물학회 2019 Journal of Plant Biology Vol.62 No.3
Pumpkin (Cucurbita maxima) fruit is the importantdietary source of carotenoid and is known for the goodflavour and texture due to the accumulation of sugar andstarch. However, lack of transcriptional information hindersour understanding of the molecular mechanisms underlyingfruit quality attributes and nutrition in C. maxima. To provideinsight into transcriptional regulation of fruit quality formationof C. maxima, quality analysis and high-throughput RNAsequencing of fruits at different developing stages werecharacterized. The quality analyses consist of dry mattervalues, percent soluble solids, carotenoid contents, and starchand sugar contents in seven stages of fruit development. Fruit transcriptome of C. maxima at five stages throughoutdevelopment was assembled to elucidate the molecularregulation of fruit development. Almost 18 billion nucleotidebases were sequenced in total, and 48,471 unigenes weredetected. A total of 32,397 (66.8%) unigenes were identifiedto be differentially expressed. We found there was a correlationbetween ripening-associated transcripts and metabolites andthe functions of regulating genes. KEGG analysis showedthere are multiple transcripts enriched in starch, sugar, carotenoid,plant hormone signal transduction and pectin pathways andseveral pathways regulating quality formation were identified. Candidate genes involving in sugar, starch, pectin, fruitsoftening and carotenoid metabolism in fruit were firstlyidentified for the species of C. maxima. Combining the sugar, starch and carotenoid accumulating patterns duringfruit development, a series of possible rate limiting geneswere identified. These findings will provide valuable informationfor further studies regarding fruit quality and development.