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- 저자 : Shin-ichi Nakayama (검색결과 4 건)
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Kimura, Satoshi,Nakayama, Kei,Wada, Masahisa,Kim, Ung-Jin,Azumi, Kaoru,Ojima, Takao,Nozawa, Akino,Kitamura, Shin-Ichi,Hirose, Euichi Inter-Research 2015 Diseases of aquatic organisms Vol.116 No.2
<P>Soft tunic syndrome is a fatal disease in the edible ascidian Halocynthia roretzi, causing serious damage to ascidian aquaculture in Korea and Japan. In diseased individuals, the tunic, an integumentary extracellular matrix of ascidians, softens and eventually tears. This is an infectious disease caused by the kinetoplastid flagellate Azumiobodo hoyamushi. However, the mechanism of tunic softening remains unknown. Because cellulose fibrils are the main component of the tunic, we compared the contents and structures of cellulose in healthy and diseased tunics by means of biochemical quantification and X-ray diffractometry. Unexpectedly, the cellulose contents and structures of cellulose microfibrils were almost the same regardless of the presence or absence of the disease. Therefore, it is unlikely that thinning of the microfibrils occurred in the softened tunic, because digestion should have resulted in decreases in crystallinity index and crystallite size. Moreover, cellulase was not detected in pure cultures of A. hoyamushi in biochemical and expressed sequence tag analyses. These results indicate that cellulose degradation does not occur in the softened tunic.</P>
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Fitness function for evolutionary system to predict unknown gene regulatory networks
Tetsuya Maeshiro,Katsunori Shimohara,Shin-ichi Nakayama 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper proposes a method, denoted pulse flux analysys, to evaluate computationally generated gene regulatory networks, without using biological knowledge related to the target gene regulatory network. Furthermore, the quantification of networks enables the ranking of predicted gene regulatory networks, and prioritization of network candidates to examine by biological experiments is also possible. A short pulse is injected to input nodes of the target network, and the response behavior is analyzed. The method also detects logical ambiguities that cannot be revealed by methods that analyze the static structure of networks. The presented method is incorporated into our system to predict gene regulatory networks, which relies on evolutionary mechanism and high simulation speed.
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Gold nanoparticles supported on mesoporous iron oxide for enhanced CO oxidation reaction
Tanaka, Shunsuke,Lin, Jianjian,Kaneti, Yusuf Valentino,Yusa, Shin-ichi,Jikihara, Yohei,Nakayama, Tsuruo,Zakaria, Mohamed Barakat,Alshehri, Abdulmohsen Ali,You, Jungmok,Hossain, Md. Shahriar A.,Yamauch The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.10
<P>Herein, we report the synthesis of gold (Au)-loaded mesoporous iron oxide (Fe2O3) as a catalyst for both CO and NH3 oxidation. The mesoporous Fe2O3 is firstly prepared using polymeric micelles made of an asymmetric triblock copolymer poly(styrene-<I>b</I>-acrylic acid-<I>b</I>-ethylene glycol) (PS-<I>b</I>-PAA-<I>b</I>-PEG). Owing to its unique porous structure and large surface area (87.0 m<SUP>2</SUP> g<SUP>−1</SUP>), the as-prepared mesoporous Fe2O3 can be loaded with a considerably higher amount of Au nanoparticles (Au NPs) (7.9 wt%) compared to the commercial Fe2O3 powder (0.8 wt%). Following the Au loading, the mesoporous Fe2O3 structure is still well-retained and Au NPs with varying sizes of 3-10 nm are dispersed throughout the mesoporous support. When evaluated for CO oxidation, the Au-loaded mesoporous Fe2O3 catalyst shows up to 20% higher CO conversion efficiency compared to the commercial Au/Fe2O3 catalyst, especially at lower temperatures (25-150 °C), suggesting the promising potential of this catalyst for low-temperature CO oxidation. Furthermore, the Au-loaded mesoporous Fe2O3 catalyst also displays a higher catalytic activity for NH3 oxidation with a respectable conversion efficiency of 37.4% compared to the commercial Au/Fe2O3 catalyst (15.6%) at 200 °C. The significant enhancement in the catalytic performance of the Au-loaded mesoporous Fe2O3 catalyst for both CO and NH3 oxidation may be attributed to the improved dispersion of the Au NPs and enhanced diffusivity of the reactant molecules due to the presence of mesopores and a higher oxygen activation rate contributed by the increased number of active sites, respectively.</P>
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Motokawa, Shogo,Narasaki, Yukie,Song, Jun-Young,Yokoyama, Yoshihiro,Hirose, Euichi,Murakami, Shoko,Jung, Sung-Ju,Oh, Myung-Joo,Nakayama, Kei,Kitamura, Shin-Ichi Elsevier 2018 Parasitology international Vol.67 No.2
<P><B>Abstract</B></P> <P>The ciliate <I>Miamiensis avidus</I> causes scuticociliatosis in Japanese flounder <I>Paralichthys olivaceus</I>. We previously reported three serotypes of this ciliate distinguishable by serotype-specific antigenic polypeptides (serotype I, 30kDa; serotype II, 38kDa; serotype III, 34kDa). In this study, we determined the localization site of the serotype-specific polypeptides in the ciliate and determined the genes encoding the polypeptides, using the isolates IyoI (serotype I), Nakajima (serotype II), and Mie0301 (serotype III). SDS-PAGE and immunoblot analysis of cilia, membrane proteins, and cytoskeletal elements of the ciliates revealed that the polypeptides were abundant in the former two. Scanning electron microscopy of ciliates immobilized by homologous antiserum showed morphological changes in the cilia. These evidences suggested that the polypeptides were ciliary membrane immobilization antigens. The ciliary genes identified showed low identity scores—<51.5% between serotypes. To differentiate the serotypes, we designed serotype-specific PCR primer sets based on the DNA sequences. The PCR-based serotyping results were completely consistent with conventional serotyping methods (immobilization assay and immunoblot analysis). Twenty of 21 isolates were classified as either serotype I or II, and one isolate was undistinguishable. The combination of species-specific PCR previously reported and three serotype-specific PCR could be useful for identifying, serotyping, and surveillance for occurrences of new serotypes of <I>M. avidus</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three serotypes of <I>M. avidus</I> were reported in Japan and Korea. </LI> <LI> Serotype-specific polypeptides were identified as ciliary membrane proteins. </LI> <LI> The nucleotide sequences of ORFs were determined. </LI> <LI> Serotype-specific PCR revealed that the pandemic serotypes were serotype I and II. </LI> </UL> </P>
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